Challenging the model for induction of metallothionein gene expression
Challenging the model for induction of metallothionein gene expression
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DOI：
10.1016/j.biochi.2006.07.021
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发表时间：
2006-11-01
期刊：
影响因子：
3.9
通讯作者：
Moreau, J. -L.
中科院分区：
文献类型：
--
作者：
Bourdineaud, J. -P.;Baudrimont, M.;Moreau, J. -L.
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Metal lothioneins (MTs) are low-molecular-weight, cysteine-rich metal-binding proteins found in a wide variety of organisms including bacteria, fungi and all eukaryotic plant and animal species. MTs bind essential and non-essential heavy metals. In mammalian cells NIT genes are highly inducible by many heavy metals including Zn, Cd, Hg, and Cu. Aquatic systems are contaminated by different pollutants, including metals, as a result of man's activities. Bivalve molluscs are known to accumulate high concentrations of heavy metals in their tissue and are widely used as bioindicators for pollution in marine and freshwater environments, with MTs frequently used as a valuable marker of metal contamination. We here describe the MT isoform gene expression patterns of marine and freshwater molluscs and fish species after Cd or Zn contamination. Contamination was carried out at a river site polluted by a zinc ore extraction plant or in the laboratory at low, environmentally relevant metal concentrations. A comparison for each species based on the accumulated MT protein levels often shows discrepancies between gene expression and protein level. In addition, several differences observed in the pattern of MT gene expression between mollusc and mammalian species enable us to discuss and challenge a model for the induction of MT gene expression. (c) 2006 Elsevier Masson SAS. All rights reserved.