Screening of native fungal strains from Yaqui Valley, producers of lignolytic enzymes
Screening of native fungal strains from Yaqui Valley, producers of lignolytic enzymes
复制标题
DOI：
10.21640/ns.v9i19.893
复制
发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Balderas-Cortés, J.J.
中科院分区：
文献类型：
--
作者：
Maldonado-Bustamante, S.R.;Mondaca-Fernández, I.;Balderas-Cortés, J.J.
摘要简介：木质纤维素由纤维素、半纤维素和木质素三种聚合物组成。生物制浆是在制浆前使用真菌作为脱木质素的手段对木质纤维素进行预处理，因为真菌可能含有酶复合物，包括木质素过氧化物酶、漆酶、锰过氧化物酶。天然真菌可具有使木质纤维素材料如小麦秸杆、木材等脱木质素的能力。 方法：本研究使用来自微生物资源生物技术实验室（索诺拉技术研究所）的COLMENA保藏的10种菌株。通过在14天的温育期间测量LiP、Lac、Xil的酶活性晕，并使用用于计算酶势指数的软件Image J 1.44确定与酶活性晕面积相对于真菌生长晕相关的酶指数，来进行菌株的选择。 结果如下：对10株菌株进行了筛选，筛选出最佳菌株为TSM 35，其潜力指数为3.83±0.29，最佳菌株为TSO 46，其潜力指数分别为20.37±2.70和1.38±0.00。通过这项研究，有可能确定在脱木质素（生物制浆）的预处理过程中引入天然真菌的潜力。 讨论或结论：从10株菌株中筛选出3株具有木质素降解活性的菌株，它们分别分泌木质素过氧化物酶、乳糖酶和木聚糖酶，具有应用于脱木质素预处理的潜力。
Abstract Introduction: Lignocellulose consists of three polymers: cellulose, hemicellulose and lignin. Biopulping is a pretreatment for lignocellulose using fungi as a means for delignifying it previously to pulping, since fungi may content an enzyme complex including lignin peroxidase, laccase, manganese peroxidase. Native fungi may have the ability to delignify lignocellulosic material such as wheat straw, wood and others. Method: This work used ten strains from COLMENA collection at the Microbial Resource Biotechnology Laboratory (Instituto Tecnologico de Sonora). The selection of strains was performed by measuring the halo of enzyme activity of LiP, Lac, Xil during 14 days of incubation and determination of enzymatic index relating the area of enzymatic activity halo against the fungi growth halo, using the software Image J 1.44 for the calculation of the enzymatic potential index. Results: Ten strains were evaluated and the best strain for LiP was TSM35 with potential index of 3.83±0.29, for Lac and Xil the strain was TSO46 with potential index of 20.37±2.70 and 1.38±0.00 respectively. With this study it is possible determine the potential for incorporate native fungi in a pretreatment process for delignification (biopulping). Discussion or Conclusion: From ten strains studied, three strains had ligninolytic activity by excretion lignin peroxidase, lacase and xylanase and they have the potential to be used in pretreatments in a delignification process.