Identification of natural rubber degradation gene in Rhizobacter gummiphilus NS21
Identification of natural rubber degradation gene in Rhizobacter gummiphilus NS21
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DOI：
10.1080/09168451.2016.1263147
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发表时间：
2017-03-01
影响因子：
1.6
通讯作者：
Fukuda, Masao
中科院分区：
文献类型：
--
作者：
Kasai, Daisuke;Imai, Shunsuke;Fukuda, Masao
革兰氏阴性橡胶降解菌——嗜胶根杆菌NS21在脱蛋白天然橡胶（DPNR）覆盖的琼脂培养基上生长并产生醛代谢物，形成一个清除区。我们分离了一个失去降解DPNR能力的转座子插入突变体，以鉴定橡胶降解基因。测序分析表明，该转座子插入了一个假定的加氧酶基因latA。推导出的氨基酸序列latA与编码黄单胞菌菌株35Y橡胶加氧酶的roxA具有36%的同源性。系统发育分析表明，LatA与RoxA构成不同的类群。在甲基libium宿主中的异源表达和latA的缺失分析表明，latA产物负责DPNR的解聚。定量反转录pcr分析表明，在DPNR生长过程中诱导了latA的转录。这些结果强烈表明，latA直接参与了NS21中橡胶的降解。
A Gram-negative rubber-degrading bacterium, Rhizobacter gummiphilus NS21 grew and produced aldehyde metabolites on a deproteinized natural rubber (DPNR)-overlay agar medium forming a clearing zone. A transposon-insertion mutant, which had lost the ability to degrade DPNR, was isolated to identify the rubber degradation genes. Sequencing analysis indicated that the transposon was inserted into a putative oxygenase gene, latA. The deduced amino acid sequence of latA has 36% identity with that of roxA, which encodes a rubber oxygenase of Xanthomonas sp. strain 35Y. Phylogenetic analysis revealed that LatA constitutes a distinct group from RoxA. Heterologous expression in a Methylibium host and deletion analysis of latA indicated that the latA product is responsible for the depolymerization of DPNR. The quantitative reverse transcription-PCR analysis indicated that the transcription of latA is induced during the growth on DPNR. These results strongly suggest that latA is directly involved in the degradation of rubber in NS21.