Development of integrated smartphone/resistive biosensor for on-site rapid environmental monitoring of organophosphate pesticides in food and water.
Development of integrated smartphone/resistive biosensor for on-site rapid environmental monitoring of organophosphate pesticides in food and water.
复制标题
开发集成智能手机/电阻生物传感器，用于食品和水中有机磷农药的现场快速环境监测。
DOI：
10.1016/j.biosx.2023.100402
复制
发表时间：
2023
影响因子：
--
通讯作者：
Wang,Jun
中科院分区：
文献类型：
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作者：
Maanaki,Hussian;Xu,Terry;Chen,Guibing;Du,Xiuxia;Wang,Jun
有机磷（OP）农药由于其急性或慢性中毒以及在世界各地农业中的广泛使用，仍然是一个全球性的健康问题。有必要为现场检测食品和水中的有机磷农药的强大和现场部署的工具。在此，我们提出了一种集成的智能手机/电阻式生物传感器，用于简单，快速，无试剂和灵敏地监测食品和环境水中的OP农药。该生物传感器利用乙酰胆碱酯酶（AChE）对其底物乙酰胆碱（ACh）的水解活性，以及壳聚糖/AChE/聚苯胺纳米纤维（PAnNF）/碳纳米管（CNT）纳米复合膜在金叉指电极上的独特传输特性。该传感器的原理依赖于OP抑制AChE，从而降低ACh水解的速率，并因此降低质子掺杂PAnNF的速率。这种导致的PAnNF电导的降低可用于定量样品中的OP农药。为生物传感器开发了一个移动的应用程序，用于分析测量数据并显示和共享测试结果。在最佳条件下，该传感器对甲基对氧磷（PM）的线性范围较宽（1 ppt ~ 100 ppb），检出限较低（0.304ppt），重现性好（RSD <5%）。此外，该生物传感器成功地应用于分析PM加标食品/水样品的平均回收率为98.3%，并提供了与液相色谱-质谱法相当的结果。因此，纳米传感平台为食品和环境水中OP农药的现场快速和灵敏检测提供了一种有前途的工具。
Organophosphate (OP) pesticides remain a worldwide health concern due to their acute or chronic poisoning and widespread use in agriculture around the world. There is a need for robust and field-deployable tools for onsite detection of OP pesticides in food and water. Herein, we present an integrated smartphone/resistive biosensor for simple, rapid, reagentless, and sensitive monitoring of OP pesticides in food and environmental water. The biosensor leverages the hydrolytic activity of acetylcholinesterase (AChE) to its substrate, acetylcholine (ACh), and unique transport properties of polyaniline nanofibers (PAnNFs) of chitosan/AChE/PAnNF/carbon nanotube (CNT) nanocomposite film on a gold interdigitated electrode. The principle of the sensor relies on OP inhibiting AChE, thus, reducing the rate of ACh hydrolysis and consequently decreasing the rate of protons doping the PAnNFs. Such resulted decrease in conductance of PAnNF can be used to quantify OP pesticides in a sample. A mobile app for the biosensor was developed for analyzing measurement data and displaying and sharing testing results. Under optimal conditions, the biosensor demonstrated a wide linear range (1 ppt–100 ppb) with a low detection limit (0.304 ppt) and high reproducibility (RSD <5%) for Paraoxon-Methyl (PM), a model analyte. Furthermore, the biosensor was successfully applied for analyzing PM spiked food/water samples with an average recovery rate of 98.3% and provided comparable results with liquid chromatography-mass spectrometry. As such, the nanosensing platform provides a promising tool for onsite rapid and sensitive detection of OP pesticides in food and environmental water.