Molecular phylogeny of Escherichia coli isolated from clinical samples in Hilla, Iraq
Molecular phylogeny of Escherichia coli isolated from clinical samples in Hilla, Iraq
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DOI：
10.5897/ajb11.1273
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发表时间：
2011-11-09
影响因子：
--
通讯作者：
Abdul-Lateef, Lamees A.
中科院分区：
文献类型：
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作者：
Abdul-Razzaq, Mohammad S.;Abdul-Lateef, Lamees A.
大肠杆菌菌株可归属于主要系统发育群之一（A、B1、B2 和 D）。这些组的菌株在表型特征上有所不同，包括使用某些糖的能力、抗生素耐药性和生长速率-温度关系。通过标准细菌学方法从不同的临床样本中总共获得了 45 株大肠杆菌分离株。通过针对两个基因 chuA、yjaA 和匿名 DNA 片段 TspE4.C2 进行 PCR 来确定这些分离株的系统发育分组。使用了三个系统发育遗传标记（chuA、yjaA 和 TspE4.C2）。结果发现，大多数大肠杆菌分离株属于系统发育A组（44.4%），包括：尿液（3个样本）、粪便（8个样本）、直肠（6个样本）和阴道（3个样本），其次是B1组（22.2%），包括尿液（4个样本）、粪便（2个样本）、直肠（3个样本）和阴道（1个样本），B2组（17.7%）包括尿液（5个样本）。样本）和阴道样本（3 个样本），而 D 组（15.5%）包括尿液样本（3 个样本）、直肠样本（1 个样本）和阴道样本 3 个。根据之前恢复的数据进行系统发育谱系分析。这项研究解释了大肠杆菌分离株在系统发育群（A、B1、B2 和 D）中的分布随宿主群体的特征以及细菌来源的变化而变化。
Escherichia coli strains can be assigned to one of the main phylogenetic groups (A, B1, B2 and D). Strains of these groups differ in their phenotypic characteristics, including the ability to use certain sugars, antibiotic resistance profiles and growth rate-temperature relationships. A total of 45 E. coli isolates were obtained from different clinical samples by standard bacteriological methods. PCR was conducted to determine the phylogenetic grouping of these isolates by targeting two genes, chuA, yjaA and anonymous DNA fragment TspE4.C2. Three phylogeny genetic markers (chuA, yjaA and TspE4.C2) were used. Results found that the most isolates of E. coli belong to the phylogeny group A (44.4%) which includes: urine (3 samples), stool (8 samples), rectal (6 samples), and vagina (3 samples), followed by group B1 (22.2%) which included urine (4 samples), stool (2 samples), rectal (3 samples) and vagina (1 samples), group B2 (17.7%) which included urine (5 samples), and vagina (3 samples), while group D (15.5%) which included urine (3 samples), rectal (1 sample) and 3 vagina samples. Phylogeny pedigree was done according to the data recovered previously. This study explains that the distributions of E. coli isolates in phylogenetic groups (A, B1, B2 and D) varied depending on the characteristics of host population and also on the variation in bacterial sources.