EXTRACELLULAR ANTIGENS OF NOCARDIA ASTEROIDES. I. PRODUCTION AND IMMUNOLOGIC CHARACTERIZATION.
EXTRACELLULAR ANTIGENS OF NOCARDIA ASTEROIDES. I. PRODUCTION AND IMMUNOLOGIC CHARACTERIZATION.
复制标题
小行星诺卡氏菌的细胞外抗原。
DOI：
--
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
R. Keeler
中科院分区：
文献类型：
--
作者：
A. Pier;R. Keeler
制备型不连续聚丙烯酰胺电泳被证明是从小行星诺卡氏菌菌株 43N 培养物硝酸盐中分离和回收凝胶沉淀素和红细胞致敏抗原的有效方法。迁移最快的级分是高度色素沉着的、电泳均质的，并且既不包含红细胞敏化活性也不包含凝胶沉淀素活性。第二快速迁移级分没有红细胞致敏活性，但显示出显着的凝胶沉淀素活性。通过电泳对该样品进行折射，得到具有一条沉淀素线的实体。分析丙烯酰胺凝胶中存在三个区域证明该材料不均匀。红细胞致敏抗原是缓慢迁移的成分，并且在大部分材料从柱中去除后被回收。尽管没有分离出所有的红细胞敏化活性，但从一个想法中回收了活性增加的部分......
Preparative, discontinuous, polyacrylamide electrophoresis was shown to be an effective method for the fractionation and recovery of gel precipitin and erythrocyte-sensitizing antigens from the culture nitrate of Nocardia asteroides strain 43N. The fastest migrating fraction was highly pigmented, electrophoretically homogenous, and contained neither erythrocyte-sensitizing activity nor gel precipitin activity. The second rapidly migrating fraction had no erythrocyte sensitizing activity, but displayed significant gel precipitin activity. Refractionation of this sample by electrophoresis resulted in an entity with one precipitin line. The material was not homogenous as evidenced by the presence of three zones in analytic acrylamide gel. The erythrocyte-sensitizing antigens were slow migrating components and were recovered after most of the material was removed from the column. Although all of the erythrocyte-sensitizing activity was not isolated, a fraction with increased activity was recovered from an ide...