Intranasal immunization with liposome-formulated Yersinia pestis vaccine enhances mucosal immune responses
Intranasal immunization with liposome-formulated Yersinia pestis vaccine enhances mucosal immune responses
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DOI：
10.1016/s0264-410x(00)00019-0
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发表时间：
2000-04-28
期刊：
影响因子：
5.5
通讯作者：
Griebel, P
中科院分区：
文献类型：
--
作者：
Baca-Estrada, ME;Foldvari, M;Griebel, P
脂质体制剂Y.鼠疫疫苗（甲脒灭活全细胞疫苗; KWC）进行了评价。我们证明了用Y.当与单独用KWC疫苗诱导的应答相比时，用脂质体配制的鼠疫KWC疫苗显著增强了肺中的粘膜免疫应答。这些免疫应答的特征在于粘膜分泌物（肺和鼻洗液）中特异性伊加和IgG的滴度增加，以及肺中特异性抗体分泌细胞的频率增加。此外，在用脂质体中配制的KWC疫苗免疫的小鼠的脾脏中，抗原特异性增殖反应和IFN-γ分泌细胞也显著增强。用KWC疫苗鼻内免疫的动物显示出对Y.鼠疫这些结果突出了粘膜施用疫苗抗原以刺激呼吸道中的免疫的重要性，并证明脂质体制剂可以提高常规疫苗的有效性。(C)2000爱思唯尔科技有限公司版权所有。
The induction of mucosal immune responses by a liposome-formulated Y. pestis vaccine (formaldehyde-killed whole cell vaccine; KWC) was evaluated. We demonstrated that intranasal immunization of mice with Y. pestis KWC vaccine, formulated with liposomes, significantly enhanced mucosal immune responses in the lung when compared to the responses induced with KWC vaccine alone. These immune responses were characterized by increased titres of specific IgA and IgG in mucosal secretions (lung and nasal washes), and an increased frequency of specific antibody-secreting cells in the lungs. In addition, antigen-specific proliferative responses and IFN-gamma-secreting cells were also significantly enhanced in the spleens of mice immunized with the KWC vaccine formulated in liposomes. Animals that were immunized intranasally with the KWC vaccine showed significant protection against an intranasal challenge with Y. pestis. These results highlight the importance of mucosal administration of vaccine antigens to stimulate immunity in the respiratory tract and demonstrate that liposome formulations can improve the effectiveness of conventional vaccines. (C) 2000 Elsevier Science Ltd. All rights reserved.