Global diversity in the human salivary microbiome
Global diversity in the human salivary microbiome
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DOI：
10.1101/gr.084616.108
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发表时间：
2009-04-01
期刊：
影响因子：
7
通讯作者：
Stoneking, Mark
中科院分区：
文献类型：
--
作者：
Nasidze, Ivan;Li, Jing;Stoneking, Mark
人类唾液微生物组可能在口腔疾病中发挥作用，并与人体其他部位（特别是肠道）的微生物组相互作用，但人们对唾液微生物组的正常变化知之甚少。我们分析了 120 名健康个体（全球 12 个地点各 10 个人）唾液样本中的 14,115 个部分（类似于 500 bp）16S 核糖体 RNA (rRNA) 序列。这些序列可以归属于 101 个已知的细菌属，其中 39 个以前从未在人类口腔中报道过；系统发育分析表明还存在另外 64 个未知属。个体内部和个体之间的唾液微生物组具有高度多样性，但地理结构很少。总体而言，属组成总方差的 13.5% 是由于个体之间的差异造成的，这与中性遗传标记总方差中可归因于人群之间差异的部分非常相似。对一些环境变量的调查揭示了地点之间的遗传距离与每个地点距赤道的距离之间存在显着关联。对人类唾液微生物组中所揭示的巨大多样性的进一步表征将有助于阐明其在人类健康和疾病中所发挥的作用，并有助于识别用于人类历史研究的潜在信息物种。
The human salivary microbiome may play a role in diseases of the oral cavity and interact with microbiomes from other parts of the human body (in particular, the intestinal tract), but little is known about normal variation in the salivary microbiome. We analyzed 14,115 partial (similar to 500 bp) 16S ribosomal RNA (rRNA) sequences from saliva samples from 120 healthy individuals (10 individuals from each of 12 worldwide locations). These sequences could be assigned to 101 known bacterial genera, of which 39 were not previously reported from the human oral cavity; phylogenetic analysis suggests that an additional 64 unknown genera are present. There is high diversity in the salivary microbiome within and between individuals, but little geographic structure. Overall, similar to 13.5% of the total variance in the composition of genera is due to differences among individuals, which is remarkably similar to the fraction of the total variance in neutral genetic markers that can be attributed to differences among human populations. Investigation of some environmental variables revealed a significant association between the genetic distances among locations and the distance of each location from the equator. Further characterization of the enormous diversity revealed here in the human salivary microbiome will aid in elucidating the role it plays in human health and disease, and in the identification of potentially informative species for studies of human population history.