Effects of denervation on the distribution of peanut agglutinin binding molecules in frog muscles.
Effects of denervation on the distribution of peanut agglutinin binding molecules in frog muscles.
复制标题
去神经对青蛙肌肉中花生凝集素结合分子分布的影响。
DOI：
10.1007/bf01187135
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Ko,CP
中科院分区：
文献类型：
--
作者：
Somasekhar,T;Ko,CP
The extracellular matrix has been shown to play an important role in the differentiation of neuromuscular junctions during reinnervation in frogs. Peanut agglutinin, a lectin, is known to specifically bind to some glycoconjugates in the extracellular matrix at the frog neuromuscular junction and myotendinous junction. In order to determine if innervation has any role in regulating the specific binding of peanut agglutinin at neuromuscular junctions and myotendinous junctions, the distribution of peanut agglutinin binding was examined in muscles chronically denervated for various periods. Short-term denervated muscles (⩽2 months) showed no changes in peanut binding agglutinin binding at neuromuscular junctions and no extrajunctional binding. In contrast, long-term denervation (>2 months-7.5 months) resulted in altered peanut agglutinin distribution and a substantial reduction or a total loss in its binding at denervated neuromuscular junctions; binding at myotendinous junctions was not affected. Results of electron microscopic studies suggest that the presence of Schwann cells at denervated endplates delays the loss of peanut agglutinin binding. Reinnervation restores normal peanut agglutinin binding at neuromuscular junctions following long-term denervation. This report demonstrates that although the distribution of peanut agglutinin binding molecules is unchanged following short-term denervation, intact innervation is necessary for the long-term maintenance of these molecules at neuromuscular junctions.
