Opisthorchis viverrini antigen induces the expression of Toll-like receptor 2 in macrophage RAW cell line
Opisthorchis viverrini antigen induces the expression of Toll-like receptor 2 in macrophage RAW cell line
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DOI：
10.1016/j.ijpara.2005.02.003
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发表时间：
2005-05-01
影响因子：
4
通讯作者：
Kawanishi, S
中科院分区：
文献类型：
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作者：
Pinlaor, S;Tada-Oikawa, S;Kawanishi, S
维韦里尼后睾吸虫感染会引起胆管内及其周围的炎症，导致人类胆管癌。为了研究 O. viverrini 诱导的炎症反应的机制，我们评估了用 O. viverrini 抗原提取物处理的 RAW 264.7 巨噬细胞系中 Toll 样受体 (TLR) 的表达。流式细胞术和免疫细胞化学显示 O. viverrini 抗原诱导 TLR2 的表达，但不诱导 TLR4 的表达。蛋白质印迹和免疫细胞化学显示，用 O. viverrini 抗原处理的 RAW 264.7 细胞中，核因子 kappa B (NF-kappa B)、诱导型一氧化氮合酶 (iNOS) 和环氧合酶-2 (COX-2) 表达呈剂量依赖性。这些结果表明 O. viverrini 通过 TLR2 介导的途径诱导炎症反应，导致 NF-κ B 介导的 NOS 和 COX-2 表达。 (C) 2005 年澳大利亚寄生虫学协会。由 Elsevier Ltd 出版。保留所有权利。
Opisthorchis viverrini infection induces inflammation in and around the bile duct, leading to cholangiocarcinoma in humans. To examine the mechanism of O. viverrini-induced inflammatory response, we assessed the expression of Toll-like receptors (TLRs) in RAW 264.7 macrophage cell line treated with an extract of O. viverrini antigen. Flow cytometry and immunocytochemistry showed that O. viverrini antigen induced the expression of TLR2 but not TLR4. Western blotting and immunocytochemistry revealed that nuclear factor-kappa B (NF-kappa B), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were expressed in RAW 264.7 cells treated with O. viverrini antigen in a dose-dependent manner. These results suggest that O. viverrini induces inflammatory response through TLR2-mediated pathway leading to NF-kappa B-mediated expression of NOS and COX-2. (C) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.