Cytoprotection by interleukin-6 against liver injury induced by lipopolysaccharide.
Cytoprotection by interleukin-6 against liver injury induced by lipopolysaccharide.
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DOI：
10.3892/ijmm.15.2.221
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发表时间：
2005-02
影响因子：
5.4
通讯作者：
Ken-ichiro Inoue;H. Takano;A. Shimada;T. Morita;R. Yanagisawa;M. Sakurai;Masahiko Sato;S. Yoshino;T. Yoshikawa
中科院分区：
文献类型：
--
作者：
Ken-ichiro Inoue;H. Takano;A. Shimada;T. Morita;R. Yanagisawa;M. Sakurai;Masahiko Sato;S. Yoshino;T. Yoshikawa
白细胞介素（IL）-6在炎症损伤中的作用仍存在争议。本研究阐明了IL-6在腹腔内注射脂多糖（LPS; 1 mg/kg）诱导的严重炎症过程中在肝损伤中的作用，使用IL-6 null（-/-）小鼠和相应的野生型（WT）小鼠。组织学研究显示，LPS处理组肝细胞损伤较IL-6（-/-）组严重，肝细胞小叶中心空泡化，肝细胞嗜中性粒细胞浸润;而LPS处理组WT组肝细胞嗜中性粒细胞浸润，肝细胞轻度空泡化。LPS刺激后，IL-6（-/-）小鼠肝脏中促炎分子（如IL-1 β、巨噬细胞炎性蛋白-1 α和巨噬细胞趋化蛋白-1）的蛋白水平显著高于WT小鼠。这些结果直接表明，IL-6对细菌内毒素诱导的肝损伤具有保护作用，至少部分是通过调节促炎细胞因子和趋化因子。
The role of interleukin (IL)-6 in inflammatory injury remains controversial. The present study elucidated the role of IL-6 in liver damage during severe inflammation induced by intraperitoneal administration of lipopolysaccharide (LPS; 1 mg/kg) using IL-6 null (-/-) mice and corresponding wild-type (WT) mice. Histological study showed that LPS treatment caused more severe liver injury with centrilobular vacuolation of hepatocytes and neutrophilic infiltration in the liver of IL-6 (-/-) mice; in contrast, neutrophilic infiltration and mild vacuolar change of hepatocytes were found in the liver of LPS-treated WT mice. Protein levels of proinflammatory molecules, such as IL-1beta, macrophage inflammatory protein-1alpha, and macrophage chemoattractant protein-1, in the livers were significantly greater in IL-6 (-/-) mice than in WT mice after LPS challenge. These results directly indicate that IL-6 is protective against liver injury induced by bacterial endotoxin, at least partly, via the modulation of proinflammatory cytokines and chemokines.