Statherin, dermcidin および Tamm-Horsfall protein を指標としたELISA 法による唾液，汗および尿の体系的識別法の開発
Statherin, dermcidin および Tamm-Horsfall protein を指標としたELISA 法による唾液，汗および尿の体系的識別法の開発
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以 Statherin、dermcidin 和 Tamm-Horsfall 蛋白为指标，使用 ELISA 开发唾液、汗液和尿液的系统鉴定方法
DOI：
10.3408/jafst.16.1
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
宏一 櫻田
中科院分区：
文献类型：
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作者：
智子 阿久津;賢一 渡邊;久代 福島;良仁 藤浪;宏一 櫻田
法医生物检材中体液的鉴定是刑事侦查中的一个重要环节。本研究建立了唾液、汗液和尿液中statherin（STATH）、dermcidin（DCD）和Tamm-Horsfall蛋白（THP）的ELISA检测方法。此外，还检查了该方法是否适用于体液污渍、老化污渍和模拟案件样本。并将ELISA检测STATH、DCD和THP的灵敏度分别与唾液、汗液和尿液的推定试验进行比较。结果表明，本研究建立的ELISA系统方法成功地鉴定了唾液、汗液和尿液。STATH在2.5岁的唾液污渍和模拟案例样本（如烟头）中成功检测到。通过ELISA的STATH的检测限（LOD）等于31.3nl唾液。虽然高于蓝淀粉琼脂糖试验，但也足以应用于个案标本。DCD在7岁的汗渍和模拟案件样本（如手机表面）中被成功检测到。ELISA法检测DCD的LOD相当于汗液的31.3 nl，与Determiner-LA试验的LOD几乎相似。在4岁儿童尿斑中成功检测出THP。ELISA法检测THP的LOD相当于9.8 nl尿液，与尿素和尿酸的推定试验结果几乎相似。结论：建立的检测唾液、汗液和尿液中STATH、DCD和THP的ELISA方法具有特异性和敏感性，可作为法医学鉴定唾液、汗液和尿液中STATH、DCD和THP的有效方法。应用ELISA检测STATH、DCD和THP，以补充唾液、汗液和尿液的推定检测。
Identification of body fluids from forensic biological samples is an essential procedure in a criminal investigation. In this study, a systematic method using ELISA for the detection of statherin (STATH), dermcidin (DCD) and Tamm-Horsfall protein (THP) has been developed to identify saliva, sweat and urine, respectively. In addition, it was examined whether this method is suitable for use on body fluid stains, aged stains, and simulated casework samples. Furthermore, the sensitivities of ELISA for STATH, DCD and THP were compared with those of presumptive tests for saliva, sweat and urine, respectively. As a result, saliva, sweat and urine were successfully identified by using the systematic method of ELISA developed in this study. STATH was successfully detected in 2.5-year-old saliva stains and simulated casework samples such as a cigarette butt. Limit of detection (LOD) of STATH by ELISA equated to 31.3 nl of saliva. It would be enough to apply to casework samples although higher than that of blue starch-agarose test. DCD was successfully detected in 7-year-old sweat stains and simulated casework samples such as the surface of a cellular phone. LOD of DCD by ELISA equated to 31.3 nl of sweat, and it was almost similar to that of the Determiner-LA test. THP was successfully detected in 4-year-old urine stains. LOD of THP by ELISA equated to 9.8 nl of urine, and it was almost similar to those of presumptive tests for urea and uric acid. In conclusion, ELISA for the detection of STATH, DCD and THP could be an effective tool for the forensic identification of saliva, sweat and urine because of its specificity and sensitivity. ELISA for the detection of STATH, DCD and THP should be applied to supplement presumptive tests for saliva, sweat and urine.
灾难酶联免疫吸附测定 ABO 和 Lewis 分型的发展
DOI：
--
发表时间：
2012
期刊：
影响因子：
--
作者：
Terutaka Sagawa; Mariko Okada
通讯作者：
Mariko Okada