Fabrication of a chitosan/glucose oxidase-poly(anilineboronic acid)-Au(nano)/Au-plated Au electrode for biosensor and biofuel cell.
Fabrication of a chitosan/glucose oxidase-poly(anilineboronic acid)-Au(nano)/Au-plated Au electrode for biosensor and biofuel cell.
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DOI：
10.1016/j.bios.2011.10.045
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发表时间：
2012-01
影响因子：
12.6
通讯作者：
Y. Huang;Xiaoli Qin;Zou Li;Yingchun Fu;Cong Qin;Feng Wu;Zhaohong Su;M. Ma;Q. Xie;S. Yao;Jiming Hu
中科院分区：
文献类型：
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作者：
Y. Huang;Xiaoli Qin;Zou Li;Yingchun Fu;Cong Qin;Feng Wu;Zhaohong Su;M. Ma;Q. Xie;S. Yao;Jiming Hu
酶的固定化是构建高性能酶传感器和生物燃料电池的关键因素之一。在此，我们提出了一种新的协议，用于有效地固定的糖蛋白酶的基础上的糖蛋白中的1，2-或1，3-二醇与硼酸官能化单体的相互作用。简单地说，将葡萄糖氧化酶（GOx）和苯胺硼酸（阿坝）的混合物浇铸到镀金的Au电极表面，然后将NaAuCl 4溶液浇铸到镀金的Au电极表面，得到GOx-聚（阿坝）（PABA）-金纳米颗粒（Aunano）生物纳米复合物，然后浇铸壳聚糖（CS）并风干。在本方案中，小尺寸的Aunanoor Au亚纳米结构可以在酶分子附近或表面形成，极大地促进了酶反应的电子转移，增强了电流响应。由此制备的CS/GOx-PABA-Aunano/Au镀Au电极在第一/第二代生物传感模式中工作良好，并作为单极生物燃料电池中的生物阳极，其分析或电池功率性能上级于迄今报道的大多数类似物。
Enzyme immobilization is one of the key factors in constructing high-performance enzyme biosensors and biofuel cells (BFCs). Herein, we propose a new protocol for efficient immobilization of a glycoprotein enzyme based on the interaction of the 1, 2- or 1, 3-diols in the glycoprotein with a boronic acid functionalized monomer. Briefly, casting a mixture of glucose oxidase (GOx) and anilineboronic acid (ABA) followed by a NaAuCl4solution to an Au-plated Au electrode surface yielded a GOx–poly(ABA) (PABA)–gold nanoparticle (Aunano) bionanocomposite, and chitosan (CS) was then cast and air-dried. In the present protocol, the small-sized Aunanoor Au subnanostructures can form near/on the enzyme molecule, which greatly promotes the electron transfer of enzymatic reaction and enhances the amperometric responses. The thus-prepared CS/GOx–PABA–Aunano/Au-plated Au electrode worked well in the first-/second generation biosensing modes and as a bioanode in a monopolar biofuel cell, with analytical or cell-power performance superior to those of most analogues hitherto reported.