BIOACTIVE CARDIAC SUBSTANCES - POTENT VASORELAXANT ACTIVITY IN MAMMALIAN ATRIA
BIOACTIVE CARDIAC SUBSTANCES - POTENT VASORELAXANT ACTIVITY IN MAMMALIAN ATRIA
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DOI：
10.1126/science.6857267
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
NEEDLEMAN, P
中科院分区：
文献类型：
--
作者：
CURRIE, MG;GELLER, DM;NEEDLEMAN, P
哺乳动物心房提取物具有利钠利尿活性。在这里报告的实验中，发现心房而不是心室的提取物也会引起离体血管和非血管平滑肌制备物的松弛。心房提取物的舒张平滑肌活性具有热稳定性和浓度依赖性，并可被蛋白酶破坏。用家兔主动脉条和鸡直肠条检测心房的生物活性。通过柱色谱法将心房活动分离成具有20，000 - 30，000和<10，000的表观MW的2个峰。当注射到清醒大鼠中时，在两个峰中与平滑肌舒张活性共纯化的心房物质引起钠尿。显然，心房具有至少2种引起平滑肌松弛和钠尿排泄的肽，这表明了一种内源性的液体容量调节系统。
Mammalian atrial extracts possess natriuretic and diuretic activity. In experiments reported here it was found that atrial, but not ventricular, extract also causes relaxation of isolated vascular and nonvascular smooth muscle preparations. The smooth muscle relaxant activity of atrial extract was heat-stable and concentration-dependent and could be destroyed with protease. Rabbit aortic and chick rectum strips were used for the detection of atrial biological activity. The atrial activity was separated by column chromatography into 2 peaks having apparent MW of 20,000-30,000 and < 10,000. The atrial substance that copurified with the smooth muscle relaxant activity in both peaks caused natriuresis when injected into conscious rats. Apparently, atria possess at least 2 peptides that elicit smooth muscle relaxation and natriuresis, suggesting an endogenous system of fluid volume regulation.