APPLICATION OF POLYMERASE CHAIN-REACTION TO DETECT ANIMALS LATENTLY INFECTED WITH AGENTS OF MALIGNANT CATARRHAL FEVER
APPLICATION OF POLYMERASE CHAIN-REACTION TO DETECT ANIMALS LATENTLY INFECTED WITH AGENTS OF MALIGNANT CATARRHAL FEVER
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DOI：
10.1177/104063879400600401
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发表时间：
1994-10-01
影响因子：
1.5
通讯作者：
HEUSCHELE, WP
中科院分区：
文献类型：
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作者：
LAHIJANI, RS;SUTTON, SM;HEUSCHELE, WP
采用聚合酶链式反应（PCR）技术，从AHV-1分离物WC11 DNA中提取寡核苷酸引物，对恶性卡他热（MCF）病原菌进行检测。对多种外来反刍动物进行了检查，以确定该技术用于检测可能被潜伏感染的动物的适用性。为了将PCR结果与血清学结果联系起来，在收集淋巴细胞的同时采集每只动物的血清样本，并进行AHV-1病毒中和试验（VNA）。共检测86只mcf易感动物，比较VNA和PCR检测结果。44只动物PCR结果为阳性。其中13例经VNA检测呈阳性。VNA和PCR均呈阳性的动物均为牛羚，是AHV-1的无症状携带者，证实了引物扩增AHV-1序列的能力。除角马外的其他物种的阳性PCR结果可能代表从AHV-1相关病毒扩增的序列，这可能不会诱导能够中和VNA中使用的WC11分离物的抗体。本研究表明，PCR能够在临床MCF出现之前检测到各种圈养反刍动物群体中MCF因子的存在，从而可以更可靠地确定感染源。
Oligonucleotide primers derived from alcelaphine herpesvirus 1 (AHV-1) isolate WC11 DNA, the first identified agent of malignant catarrhal fever (MCF), were used to assay blood lymphocyte DNA using the polymerase chain reaction (PCR). Multiple species of exotic ruminants were examined to determine the suitability of this technique for detecting animals that may be latently infected. To correlate the PCR results with those of serology, serum samples were obtained from each animal concurrently with lymphocyte collection and subjected to an AHV-1 virus-neutralization assay (VNA). A total of 86 MCF-susceptible animals were tested, and the results of the VNA and PCR assays were compared. PCR results were positive for 44 animals. Of these, 13 were positive by VNA. Animals positive by both VNA and PCR were all wildebeest, the asymptomatic carriers of AHV-1, confirming the ability of the primers to amplify AHV-1 sequence. Positive PCR results from species other than wildebeest may represent sequence amplified from viruses related to AHV-1, which may not induce antibodies capable of neutralizing the WC11 isolate used in the VNA. This study demonstrates that PCR is capable of detecting the presence of MCF agents in various populations of captive ruminants prior to the appearance of clinical MCF so that the sources of infection can be more reliably ascertained.