Physiologic and Biologic Characteristics of Three Experimental Models of Acute Lung Injury in Rats
Physiologic and Biologic Characteristics of Three Experimental Models of Acute Lung Injury in Rats
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DOI：
10.1213/ane.0b013e3182104dac
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发表时间：
2011-05-01
影响因子：
5.7
通讯作者：
Zhang, Haibo
中科院分区：
文献类型：
--
作者：
Henzler, Dietrich;Hochhausen, Nadine;Zhang, Haibo
背景：减轻呼吸机相关肺损伤的策略已经在急性肺损伤（ALI）的各种实验方法中进行了测试。结论往往是从生理和生物效应，但这些结果的影响模型是未知的。方法：20只Sprague道利大鼠麻醉后，机械通气5 h。研究了三种ALI模型（表面活性剂洗脱、酸吸入和大潮气量通气）的血流动力学、呼吸力学、气体交换、肺病理学和炎症反应。结果：每组5只动物进行分析。1小时后，所有组的动态顺应性和PaO（2）/吸入氧分数比均下降至少50%。尽管在所有模型中依从性仍然降低，但灌洗组的氧合在5小时后恢复到基线值。高潮气量模型中弥漫性肺泡损伤更严重，对照组和灌洗组动物之间无差异。白细胞介素-6增加支气管肺泡灌洗液中的吸入和高潮气量models.CONCLUSIONS：虽然可比的生理效果，满足急性呼吸窘迫综合征的标准，在所有模型中实现，肺损伤模型之间的生物反应各不相同。酸吸入模型产生了呼吸和炎症反应，通常见于ALI;这些数据表明，它可能是临床上最适用的模型，以研究大鼠呼吸机相关肺损伤的中期影响。（Anesth Analg 2011;112：1139-46）
BACKGROUND: Strategies to attenuate ventilator-associated lung injury have been tested in various experimental methods of acute lung injury (ALI). Conclusions are often drawn from physiologic and biologic effects, but the influence of the model on these results is not known. Our aim in this study was to characterize frequently used models of experimental ALI.METHODS: Twenty Sprague Dawley rats were anesthetized and their lungs mechanically ventilated for 5 hours. Three models of ALI (surfactant washout, acid aspiration, and high tidal volume ventilation) were investigated with regard to hemodynamics, respiratory mechanics, gas exchange, lung pathology, and inflammatory reactions. Animals without ALI served as controls.RESULTS: Five animals in each group were analyzed. Dynamic compliance and PaO(2)/fraction of inspired oxygen ratio decreased by at least 50% in all groups after 1 hour. Whereas compliance remained decreased in all models, oxygenation returned to baseline values in the lavage group after 5 hours. Diffuse alveolar damage was worse in the high tidal volume model and was not different between the control and lavage animals. Interleukin-6 was increased in bronchoalveolar lavage fluid in the aspiration and high tidal volume models.CONCLUSIONS: Although comparable physiologic effects meeting acute respiratory distress syndrome criteria were achieved in all models, the biologic responses varied among lung injury models. The acid aspiration model created both respiratory and inflammatory responses typically seen in ALI; these data suggest that it may be the most clinically applicable model to study the intermediate-term effects of ventilator-associated lung injury in rats. (Anesth Analg 2011;112:1139-46)