TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae
TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae
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基于 TALEN 的 TFIIAy5 编辑改变了水稻对黄单胞菌 pv. 的反应。
DOI：
10.1038/s41598-020-59052-w
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发表时间：
2020-02-06
影响因子：
4.6
通讯作者：
Zhai, Wenxue
中科院分区：
文献类型：
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作者：
Han, Jin;Xia, Zhihui;Zhai, Wenxue
Xa5基因编码一种对水稻白叶枯病具有广谱抗性的碱性转录因子(TFIIA)蛋白。稻米中的稻种(Xoo)。仅在少数水稻生态型中发现，其隐性特性限制了其在育种上的应用。在这里，我们使用基于TALEN的技术编辑其显性等位基因TFIIA Gamma 5，并获得了许多突变的TFIIA Gamma 5基因。当植株受到Xoo的攻击时，它们中的大多数都不同程度地降低了水稻的感病能力。特别是TFIIA-Gamma 5基因敲除后，虽然不能达到Xa5介导的抗性水平，但仍能显著降低水稻的感病能力，说明TFIIA-Gamma 5是水稻感病的主要成分。此外，编码第32位氨基酸缺失或第32位至第33位氨基酸插入的突变体使水稻具有与敲除的TFIIA-Gamma 5类似的抗性。因此，第32位附近的氨基酸也是TFIIA-Gamma 5的重要作用位点。此外，将Xa5整合到TFIIA伽玛5基因敲除植株中，使它们具有与IRBB5相似的抗性，IRBB5是含有纯合子xa5基因的水稻品种。因此，TFIIA-Gamma 5不是简单地被认为是一个抗性或感病基因座，因为氨基酸的替代可能改变其功能。
The xa5 gene encodes a basal transcription factor (TFIIA gamma) protein with wide spectrum resistance to bacterial blight caused by Xanthomonas oryzae pv. Oryzae (Xoo) in rice. It was only found in a few rice ecotypes, and the recessive characteristics limited its application in breeding. Here, we employed a TALEN-based technique to edit its dominant allelic TFIIA gamma 5 and obtained many mutant TFIIA gamma 5 genes. Most of them reduced rice susceptibility to varying degrees when the plants were challenged with the Xoo. In particular, the knocked-out TFIIA gamma 5 can reduce the rice susceptibility significantly, although it cannot reach the xa5-mediated resistance level, indicating TFIIA gamma 5 is a major component involved in disease susceptibility. In addition, the mutant encoding the protein with deletion of the 32nd amino acid or amino acid insertion between 32nd and 33rd site confers rice with the similar resistance to that of the knocked-out TFIIA gamma 5. Thus, the amino acids around 32nd site are also the important action sites of TFIIA gamma 5 besides the 39th amino acid previously reported. Moreover, the integration of xa5 into TFIIA gamma 5-knockout plants conferred them with a similar resistance as IRBB5, the rice variety containing the homozygous xa5 gene. Thus, TFIIA gamma 5 was not simply regarded as a resistant or a susceptible locus, as the substitution of amino acids might shift its functions.