IDENTIFICATION AND CHARACTERIZATION OF A 2ND MAJOR PEANUT ALLERGEN, ARA H II, WITH USE OF THE SERA OF PATIENTS WITH ATOPIC-DERMATITIS AND POSITIVE PEANUT CHALLENGE
IDENTIFICATION AND CHARACTERIZATION OF A 2ND MAJOR PEANUT ALLERGEN, ARA H II, WITH USE OF THE SERA OF PATIENTS WITH ATOPIC-DERMATITIS AND POSITIVE PEANUT CHALLENGE
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DOI：
10.1016/0091-6749(92)90469-i
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发表时间：
1992-12-01
影响因子：
14.2
通讯作者：
HELM, RM
中科院分区：
文献类型：
--
作者：
BURKS, AW;WILLIAMS, LW;HELM, RM
花生常常是儿童食物过敏反应的一个诱因。在花生过敏原的鉴定和纯化过程中，使用了9名患有特应性皮炎且对花生的双盲、安慰剂对照食物激发试验呈阳性的患者的血清。通过使用各种生化和分子技术，完成了对第二种主要花生过敏原的鉴定。对粗花生提取物进行阴离子交换色谱分析，得到了几个组分，这些组分能与激发试验呈阳性的患者血清中的IgE结合。通过检测抗花生特异性IgE以及IgE特异性免疫印迹，我们鉴定出一种过敏成分，它有两条紧密迁移的条带，平均分子量为17 kD。该组分的双向凝胶电泳显示其平均等电点为5.2。根据国际免疫学会联合会过敏原命名小组委员会的过敏原命名法，这种过敏原被命名为Ara h II（花生）。
Peanuts are frequently a cause of food hypersensitivity reactions in children. Serum from nine patients with atopic dermatitis and a positive double-blind, placebo-controlled, food challenge to peanut were used in the process of identification and purification of the peanut allergens. Identification of a second major peanut allergen was accomplished with use of various biochemical and molecular techniques. Anion exchange chromatography of the crude peanut extract produced several fractions that bound IgE from the serum of the patient pool with positive challenges. By measuring antipeanut specific IgE and by IgE-specific immunoblotting we have identified an allergic component that has two closely migrating bands with a mean molecular weight of 17 kd. Two-dimensional gel electrophoresis of this fraction revealed it to have a mean isoelectric point of 5.2. According to allergen nomenclature of the IUIS Subcommittee for Allergen Nomenclature this allergen is designated, Ara h II (Arachis hypogaea).