Cell adhesion on NiTi thin film sputter-deposited meshes.
Cell adhesion on NiTi thin film sputter-deposited meshes.
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DOI：
10.1016/j.msec.2015.10.008
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
K. Loger;A. Engel;J. Haupt;Q. Li;R. Lima de Miranda;E. Quandt;G. Lutter;C. Selhuber‐Unkel
中科院分区：
文献类型：
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作者：
K. Loger;A. Engel;J. Haupt;Q. Li;R. Lima de Miranda;E. Quandt;G. Lutter;C. Selhuber‐Unkel
组织工程支架使得在体外制造和形成生物医学植入物成为可能，这些植入物在体内具有特殊的功能。本研究采用磁控溅射沉积的方法制备了独立的镍钛（NiTi）薄膜网。网格包含精确定义的尺寸为440 ~ 1309 μm2的方形孔，支撑宽度为5.3 ~ 9.2 μm。通过拉伸试验研究了微结构超弹性NiTi薄膜的有效力学性能。这些结果将适用于膜孔的设计。在培养24 h和7 d后，研究了孔和杆的尺寸对羊自体细胞（CD133 +）粘附的影响。荧光显微镜和扫描电镜的光学分析表明，细胞的粘附性取决于网格的结构参数。细胞培养7天后，大部分网状物被排列的纤维材料覆盖。在440 μm2的菱形小孔和5.3 μm的支撑孔上，细胞的粘附能力较强。我们的研究结果表明，独立的镍钛薄膜网格在心血管组织工程，特别是心脏瓣膜的制造中具有很好的应用潜力。
Scaffolds for tissue engineering enable the possibility to fabricate and form biomedical implants in vitro, which fulfill special functionality in vivo. In this study, free-standing Nickel–Titanium (NiTi) thin film meshes were produced by means of magnetron sputter deposition. Meshes contained precisely defined rhombic holes in the size of 440 to 1309 μm2and a strut width ranging from 5.3 to 9.2 μm. The effective mechanical properties of the microstructured superelastic NiTi thin film were examined by tensile testing. These results will be adapted for the design of the holes in the film. The influence of hole and strut dimensions on the adhesion of sheep autologous cells (CD133 +) was studied after 24 h and after seven days of incubation. Optical analysis using fluorescence microscopy and scanning electron microscopy showed that cell adhesion depends on the structural parameters of the mesh. After 7 days in cell culture a large part of the mesh was covered with aligned fibrous material. Cell adhesion is particularly facilitated on meshes with small rhombic holes of 440 μm2and a strut width of 5.3 μm. Our results demonstrate that free-standing NiTi thin film meshes have a promising potential for applications in cardiovascular tissue engineering, particularly for the fabrication of heart valves.