Self-regenerating giant hyaluronan polymer brushes
Self-regenerating giant hyaluronan polymer brushes
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DOI：
10.1038/s41467-019-13440-7
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发表时间：
2019-12
影响因子：
16.6
通讯作者：
Wenbin Wei;J. Faubel;Hemaa Selvakumar;Daniel T. Kovari;Joanna Tsao;Felipe Rivas;Amar T. Mohabir;Michelle C Krecker;Elaheh Rahbar;A. Hall;M. Filler;Jennifer L. Washburn;P. Weigel;J. Curtis
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文献类型：
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作者：
Wenbin Wei;J. Faubel;Hemaa Selvakumar;Daniel T. Kovari;Joanna Tsao;Felipe Rivas;Amar T. Mohabir;Michelle C Krecker;Elaheh Rahbar;A. Hall;M. Filler;Jennifer L. Washburn;P. Weigel;J. Curtis
用聚合物刷定制界面是为许多应用创造功能材料的常用策略。现有方法在刷厚度、产生生物聚合物的高密度刷的能力以及再生潜力方面受到限制。在这里，我们介绍了一个计划，合成超厚再生透明质酸聚合物刷使用透明质酸合酶。该平台提供了一个动态界面，可调刷高度可达20微米-比标准刷厚两个数量级。通过酶的光失活，这些刷子很容易雕刻成微图案的风景。此外，它们提供兆道尔顿透明质酸的连续来源，或者它们可以共价稳定到表面。稳定的刷子对生物膜表现出极好的抵抗力，但被成纤维细胞局部消化。这种刷技术在一系列领域提供了机会，包括用于植入物、伤口愈合或润滑的再生可定制生物界面，以及糖萼和聚合物物理学的基础研究。
Tailoring interfaces with polymer brushes is a commonly used strategy to create functional materials for numerous applications. Existing methods are limited in brush thickness, the ability to generate high-density brushes of biopolymers, and the potential for regeneration. Here we introduce a scheme to synthesize ultra-thick regenerating hyaluronan polymer brushes using hyaluronan synthase. The platform provides a dynamic interface with tunable brush heights that extend up to 20 microns – two orders of magnitude thicker than standard brushes. The brushes are easily sculpted into micropatterned landscapes by photo-deactivation of the enzyme. Further, they provide a continuous source of megadalton hyaluronan or they can be covalently-stabilized to the surface. Stabilized brushes exhibit superb resistance to biofilms, yet are locally digested by fibroblasts. This brush technology provides opportunities in a range of arenas including regenerating tailorable biointerfaces for implants, wound healing or lubrication as well as fundamental studies of the glycocalyx and polymer physics.