Use of bacterial whole-genome sequencing to investigate local persistence and spread in bovine tuberculosis
Use of bacterial whole-genome sequencing to investigate local persistence and spread in bovine tuberculosis
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DOI：
10.1016/j.epidem.2015.08.003
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发表时间：
2016-03-01
期刊：
影响因子：
3.8
通讯作者：
Kao, Rowland R.
中科院分区：
文献类型：
--
作者：
Trewby, Hannah;Wright, David;Kao, Rowland R.
牛分枝杆菌是牛结核病的病原体，这是英国养牛业目前面临的最重要的疾病之一。在这里，我们使用高密度全基因组测序（WGS）在一个定义的亚群的M。在66个牛群细分的145头牛中进行了牛病毒的研究，以深入了解当地的传播和持久性。我们发现，尽管分离物之间的分歧很小，WGS原则上可以暴露出采样不足的宿主种群对M的贡献。牛传染然而，我们证明，在我们的数据中，这样的信号是由于分子类型转换，这是以前没有记录的M。牛来自具有已知的直接牛运动历史的农场的分离株没有显示出较高遗传相似性的统计信号。尽管遗传隔离的整体信号的距离，遗传距离也没有表现出明显的关系，空间距离受影响的农场之间的距离
Mycobacterium bovis is the causal agent of bovine tuberculosis, one of the most important diseases currently facing the UK cattle industry. Here, we use high-density whole genome sequencing (WGS) in a defined sub-population of M. bovis in 145 cattle across 66 herd breakdowns to gain insights into local spread and persistence. We show that despite low divergence among isolates, WGS can in principle expose contributions of under-sampled host populations to M. bovis transmission. However, we demonstrate that in our data such a signal is due to molecular type switching, which had been previously undocumented for M. bovis. Isolates from farms with a known history of direct cattle movement between them did not show a statistical signal of higher genetic similarity. Despite an overall signal of genetic isolation by distance, genetic distances also showed no apparent relationship with spatial distance among affected farms over distances