REGULATION AND SPECIFICITY OF GLUCOSE-STIMULATED INSULIN GENE-EXPRESSION IN HUMAN ISLETS OF LANGERHANS
REGULATION AND SPECIFICITY OF GLUCOSE-STIMULATED INSULIN GENE-EXPRESSION IN HUMAN ISLETS OF LANGERHANS
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DOI：
10.1016/0014-5793(87)80523-9
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发表时间：
1987-10-19
期刊：
影响因子：
3.5
通讯作者：
GRAY, DWR
中科院分区：
文献类型：
--
作者：
HAMMONDS, P;SCHOFIELD, PN;GRAY, DWR
通过mRNA和多肽水平测定培养人朗格汉斯胰岛对自然和药物刺激的胰岛素反应。我们报告了细胞外葡萄糖对mRNA水平和胰岛素分泌的剂量依赖性刺激，并提供证据表明胰岛反应性可分为两个时间阶段：早期反应，明显受转录后控制，以及胰岛素信使积累的晚期。尽管葡萄糖对人类的影响与啮齿动物相似，但存在重要差异，特别是在β细胞蛋白激酶激活剂对葡萄糖刺激的调节方面。
The insulin response of cultured human islets of Langerhans was measured at both mRNA and polypeptide levels in response to natural and pharmacological stimuli. We report a dosage dependent stimulation of both mRNA levels and insulin secretion by extracellular glucose, and present evidence that islet responsiveness can be divided into two temporal phases: an early response, apparently under post-transcriptional control, and a late phase in which insulin messenger accumulates. Although glucose effects in man are similar to rodents, there are important differences, especially with respect to modulation of glucose stimulation by activators of β-cell protein kinases.