Immunization by application of DNA vaccine onto a skin area wherein the hair follicles have been induced into anagen-onset stage.
Immunization by application of DNA vaccine onto a skin area wherein the hair follicles have been induced into anagen-onset stage.
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通过将DNA疫苗应用到皮肤区域来进行免疫，其中毛囊已被诱导进入毛发生长初期开始阶段。
DOI：
10.1038/sj.mt.6300286
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Cui,Zhengrong
中科院分区：
文献类型：
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作者：
Shaker,DaliaS;Sloat,BrianR;Le,UyenM;Löhr,ChristianeV;Yanasarn,Nijaporn;Fischer,KayA;Cui,Zhengrong
一种有吸引力的免疫方法是将DNA疫苗局部应用于皮肤。然而，重要的是要确保在不破坏皮肤角质层的情况下诱导强烈的免疫反应。毛囊已被证明是DNA进入皮肤的主要通道，据报道，毛囊细胞的转基因主要发生在毛发周期的新的生长阶段开始。利用炭疽保护性抗原(PA)蛋白编码基因在小鼠体内的应用表明，当应用区域的毛囊被诱导进入生长期时，抗PA免疫应答明显强于端原期。抗PA抗体使免疫的小鼠能够在致命剂量的炭疽致命毒素攻击下存活下来。这种增强的免疫反应部分归因于毛囊被诱导进入生长期的皮肤区域抗原基因表达和质粒DNA摄取的增强。此外，与生长期诱导相关的中度真皮炎症也可能有助于增强所产生的免疫反应。这代表了一种增强局部应用DNA疫苗诱导的免疫反应的新方法。
An attractive approach to immunization is to apply DNA vaccine topically onto the skin. However, it is important to ensure that a strong immune response is induced without disrupting the skin stratum corneum. The hair follicles have been shown to be the major portal of entry for DNA applied onto the skin, and it has been reported that the transfection of hair follicle cells occurs mainly at the onset of a new growing stage of the hair cycle. Using an anthrax protective antigen (PA) protein-encoding plasmid in mice, we demonstrated that the anti-PA immune responses were significantly stronger when the hair follicles in the application area were induced into anagen-onset stage than when in telogen stage. The anti-PA antibodies enabled the immunized mice to survive a lethal dose of anthrax lethal toxin challenge. The enhanced immune responses can be partially attributed to the enhanced antigen gene expression and plasmid DNA uptake in the skin area wherein the hair follicles were induced into anagen-onset stage. Moreover, the moderate dermal inflammation associated with the anagen induction may also have contributed to the enhancement of the resultant immune response. This represents a novel approach to enhancing the immune response induced by a topically applied DNA vaccine.