ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE
ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE
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DOI：
10.1073/pnas.88.18.7978
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
BENKOVIC, SJ
中科院分区：
文献类型：
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作者：
BARBAS, CF;KANG, AS;BENKOVIC, SJ
为在丝状噬菌体M13表面单价展示组合抗体Fab文库，建立了一种噬菌体系统。Fab片段融合到基因III蛋白的羧基末端结构域。在表面展示Fab片段的噬菌体，或称Phab，在抗原包被的表面上比非特异性噬菌体富含10(3)-10(5)倍。该方法可能会取代目前的抗体克隆技术。
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.