Urinary high-mobility group box-1 associates specifically with lupus nephritis class V.
Urinary high-mobility group box-1 associates specifically with lupus nephritis class V.
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DOI：
10.1177/0961203316644331
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发表时间：
2016-12
期刊：
影响因子：
2.6
通讯作者：
Caricchio R
中科院分区：
文献类型：
--
作者：
Jog NR;Blanco I;Lee I;Putterman C;Caricchio R
高迁移率族蛋白 1 (HMGB-1) 与狼疮性肾炎 (LN) 的发病机制有关。 LN 患者的肾脏中 HMGB-1 表达增加，血清和尿液中的水平也增加。本研究的目的是确定尿液中 HMGB-1 的增加是否是活动性狼疮特有的，还是继发于肾损伤的。本研究纳入了 61 名狼疮患者（32 名患有活动性 LN，29 名患有 SLE，但无狼疮证据）和 14 名对照蛋白尿患者（全部患有高血压，8 名患有糖尿病）的尿液。通过蛋白质印迹法检测HMGB-1。将尿蛋白标准化为尿肌酐，以考虑样本的体积。与无肾脏疾病的狼疮患者相比，LN 患者的中位标准化尿液 HMGB-1 水平显着升高（53.81 vs. 9.46，p<0.001）。 LN 类别之间的中位水平存在差异；增殖性疾病和膜性疾病之间存在显着差异（33.4 vs. 138.8，p=0.003）。尿蛋白与尿肌酐比 (P/C) 与尿 HMGB-1 相关（r=0.52，p<0.001），但在所有类别中，这种情况仅适用于膜性疾病（r=0.71，p=0.022，增殖性，p=0.63；混合性，p=0.34）。活动性 LN 患者尿液中 HMGB-1 升高。水平与 LN 类别相关，与增殖型和混合型患者相比，V 型患者的尿 HMGB-1 水平较高。因此，尿 HMGB-1 可能提示膜性 LN，并需要在大型狼疮队列和对照疾病中进行进一步评估。
High mobility group box 1 protein (HMGB-1) has been implicated in the pathogenesis of lupus nephritis (LN). There is increased HMGB-1 expression in the kidneys and increased levels are observed in serum and urine of patients with LN. This study was performed to determine whether the increased urinary HMGB-1 was specific for active lupus or secondary to renal damage. Urine from 61 lupus patients (32 had active LN and 29 had SLE with no evidence of LN) and 14 control proteinuric patients (all with hypertension and 8 also with diabetes) were included in this study. HMGB-1 was detected by western blot. Urine protein was normalized to urine creatinine to account for volume of the specimen. Median normalized urine HMGB-1 levels were significantly elevated in LN patients compared to lupus patients without kidney disease (53.81 vs. 9.46, p<0.001). A difference in median levels was seen between LN classes; with a significant difference between proliferative and membranous disease (33.4 vs. 138.8, p=0.003). Urine protein to urine creatinine ratio (P/C) correlated with urinary HMGB-1 (r=0.52, p<0.001), but across the classes this was true only for membranous disease (r=0.71, p=0.022, proliferative, p=0.63; mixed, p=0.34). HMGB-1 is elevated in the urine of patients with active LN. Levels are associated with LN class and higher levels of urinary HMGB-1 are seen in patients with class V when compared to both proliferative and mixed classes. Therefore, urinary HMGB-1 may be suggestive of membranous LN and warrants further evaluation in large lupus cohort and control disease.
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影响因子：
4.9
作者：
Abdulahad DA;Westra J;Bijzet J;Limburg PC;Kallenberg CG;Bijl M
通讯作者：
Bijl M
影响因子：
4.4
作者：
Qing, Xiaoping;Pitashny, Milena;Thomas, David B.;Barrat, Franck J.;Hogarth, Mark P.;Putterman, Chaim
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影响因子：
13.6
作者：
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通讯作者：
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DOI：
10.1084/jem.20120189
发表时间：
2012-08-27
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Venereau E;Casalgrandi M;Schiraldi M;Antoine DJ;Cattaneo A;De Marchis F;Liu J;Antonelli A;Preti A;Raeli L;Shams SS;Yang H;Varani L;Andersson U;Tracey KJ;Bachi A;Uguccioni M;Bianchi ME
通讯作者：
Bianchi ME
DOI：
10.1097/01.asn.0000108969.21691.5d
发表时间：
2004-02-01
影响因子：
13.6
作者：
Weening, JJ;D'Agati, VD;Nagata, M
通讯作者：
Nagata, M