Evaluation of diquat against an acute experimental infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus (Rafinesque)
Evaluation of diquat against an acute experimental infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus (Rafinesque)
复制标题
DOI：
10.1111/j.1365-2761.2009.01024.x
复制
发表时间：
2009-05-01
影响因子：
2.5
通讯作者：
Mitchell, A. J.
中科院分区：
文献类型：
--
作者：
Darwish, A. M.;Mitchell, A. J.
研究了双喹特（6,7-二氢双吡啶[1,2- A:2',1'-c]吡嗪二溴）对斑点鲶鱼急性柱状黄杆菌感染的防治效果。Diquat是环境保护局批准的除草剂，有可能在法律上和实际中用于对付柱状虫。对通道鲶鱼进行皮肤磨损和水源暴露，并在攻毒后22 h分别用2.5、5.0、10.0和15 mg L-1的diquat活性成分处理一次，处理6小时。试验结束后21天，5.0、10.0和15 mg L-1的diquat活性成分显著（P < 0.05）将攻毒后未处理鱼的感染鱼死亡率从95%降低到68%、59%和49%。对23株柱状芽胞杆菌进行了体外最低抑菌浓度（MIC）测定。大多数分离株的MIC值为5 μ g mL(-1)（23株中有15株）。受感染的鱼表现出与自然感染相似的急性临床症状。皮肤有严重的溃疡性坏死性皮炎，肌肉有严重的坏死性肌炎。鳃部有严重的多灶性坏死性支炎。结果表明，diquat可降低急性柱体感染的死亡率。
A trial was performed to evaluate the efficacy of diquat (6,7-dihydrodipyrido[1,2-a:2',1'-c]pyrazinediium dibromide) against an acute experimental infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus. Diquat is an Environmental Protection Agency-approved herbicide and has the potential to be legally and practically used against columnaris. Channel catfish were challenged, by cutaneous abrasion, and waterborne exposure to F. columnare and treated once at 22-h post-challenge with 2.5, 5.0, 10.0 and 15 mg L-1 of diquat active ingredient for 6 h. At the conclusion of the trial, 21-day post-challenge, diquat at 5.0, 10.0 and 15 mg L-1 significantly (P < 0.05) reduced the mortality of infected fish from 95% in the challenged non-treated fish to 68%, 59% and 49%, respectively. In vitro, the minimum inhibitory concentration (MIC) of 23 isolates of F. columnare was assayed. The majority of the isolates had an MIC value of 5 mu g mL(-1) (15 of the 23 isolates). Infected fish exhibited acute clinical signs similar to a natural infection. The skin had severe ulcerative necrotizing dermatitis and the muscles had severe necrotizing myositis. The gills had severe multifocal necrotizing branchitis. The results demonstrate that diquat would reduce mortalities caused by an acute columnaris infection.