Antibody to a sperm surface glycoprotein inhibits the egg jelly-induced acrosome reaction of sea urchin sperm.
Antibody to a sperm surface glycoprotein inhibits the egg jelly-induced acrosome reaction of sea urchin sperm.
复制标题
精子表面糖蛋白抗体抑制卵冻诱导的海胆精子顶体反应。
DOI：
10.1016/0012-1606(80)90119-0
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发表时间：
1980
影响因子：
2.7
通讯作者：
Vacquier,VD
中科院分区：
文献类型：
--
作者：
Lopo,AC;Vacquier,VD
当海胆（Strongylocentrotus purpuratus）精子表面进行放射性碘标记时，75%的蛋白质掺入放射性与Mr 84，000（84 K）64，000（64 K）的两种糖蛋白相关（Lopo和Vacquier 1980）。通过在SDS-聚丙烯酰胺凝胶上分离精子的Triton X-100提取物，切下含有糖蛋白的条带并将均质化的凝胶注射到兔子体内，制备针对这两种成分的抗体。抗84 K和抗64 K血清均能凝集精子。光镜和电镜免疫过氧化物酶定位显示两种抗原均分布于整个精子表面。用免疫过氧化物酶技术，这两种抗血清与其他球海胆属的精子有一定程度的交叉反应，但与其他属的精子无交叉反应。与抗84 K Fab片段孵育的活精子完全抑制了卵子受精诱导的顶体反应和受精卵。抗64 K Fab片段对精子进行顶体反应或使卵子受精的能力没有影响。在抗84 K或抗64 K Fab片段中孵育的精子响应于Ca 2+离子载体A23187或当细胞外pH用NH 4 OH增加至9.2时经历顶体反应，这表明抗84 K Fab与参与顶体反应的起始或传播的外部分子结合导致对卵母细胞诱导的顶体反应的抑制。84 K糖蛋白是第一个被鉴定的精子表面成分，可能在诱导顶体反应中起作用。
When the surface of sea urchin (Strongylocentrotus purpuratus) sperm is radioiodinated, 75% of the protein-incorporated radioactivity is associated with two glycoproteins ofMr84,000 (84K) 64,000 (64K) (Lopo and Vacquier 1980). Antibodies were prepared against these two components by separating a Triton X-100 extract of sperm on SDS-polyacrylamide gels, cutting out the band containing the glycoprotein and injecting the homogenized gel into rabbits. Both anti-84K and anti-64K sera agglutinate sperm. Light and EM immunoperoxidase localization show both antigens are distributed over the entire sperm surface. By the immunoperoxidase technique there is some degree of cross-reactivity of both antisera with sperm of otherStrongylocentrotusspecies, but not with those of other genera. Living sperm incubated with anti-84K Fab fragments are completely inhibited from undergoing the egg jelly-induced acrosome reaction and fertilizing eggs. Anti-64K Fab fragments have no effect on the ability of the sperm to undergo the acrosome reaction or fertilize eggs. Sperm incubated in anti-84K or anti-64K Fab fragments undergo the acrosome reaction in response to the Ca2+ionophore A23187, or when the extracellular pH is increased to 9.2 with NH4OH, indicating that the inhibition of the egg jelly-induced acrosome reaction results from the binding of the anti-84K Fab to an external molecule involved in the initiation or propagation of the acrosome reaction. The 84K glycoprotein is the first sperm surface component identified that might have a role in the induction of the acrosome reaction.