Effect of dichloroacetate on lactate concentration in exercising humans.
Effect of dichloroacetate on lactate concentration in exercising humans.
复制标题
二氯乙酸对运动人体乳酸浓度的影响。
DOI：
10.1152/jappl.1989.66.2.591
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Wolfe,RR
中科院分区：
文献类型：
--
作者：
Carraro,F;Klein,S;Rosenblatt,JI;Wolfe,RR
人类运动期间血浆乳酸浓度增加的确切机制尚不清楚。我们使用二氯乙酸来检验丙酮酸脱氢酶活性的限制是血浆乳酸升高的原因的假设。二氯乙酸会刺激丙酮酸脱氢酶的活性，丙酮酸脱氢酶通常是组织氧合充足时葡萄糖氧化的调节酶。根据随机交叉方案对六名受试者进行了两次研究，其中一项测试使用盐水输注，另一项测试使用二氯乙酸盐输注。自行车测力计上的运动负荷逐渐增加直至精疲力竭。在整个运动过程中每分钟抽取一次血样，并在 120 分钟的恢复过程中定期抽取血样。在低于平均最大耗氧量 80% 的运动过程中，二氯乙酸显着降低了乳酸浓度。力竭时乳酸的峰值浓度不受二氯乙酸处理的影响，但二氯乙酸在整个恢复过程中降低了乳酸浓度。这些结果表明，丙酮酸脱氢酶活性的限制导致次最大运动和恢复期间血浆乳酸的增加。
The precise mechanism responsible for the increase in plasma lactate concentration during exercise in humans is not known. We have used dichloroacetate to test the hypothesis that a limitation in pyruvate dehydrogenase activity is responsible for the rise in plasma lactate. Dichloroacetate stimulates the activity of pyruvate dehydrogenase, which is normally the regulatory enzyme in the oxidation of glucose when tissue oxygenation is adequate. Six subjects were studied twice according to a randomized, crossover protocol, involving one test with saline infusion and another with dichloroacetate infusion. Exercise load on a bicycle ergometer was increased progressively until exhaustion. Blood samples were drawn each minute throughout exercise and periodically throughout 120 min of recovery. Dichloroacetate significantly lowered the lactate concentration during exercise performed at less than 80% of the average maximal O2 consumption. The peak concentration of lactate at exhaustion was not affected by dichloroacetate treatment, but dichloroacetate did lower lactate concentration throughout recovery. These results suggest that a limitation in pyruvate dehydrogenase activity contributes to the increase in plasma lactate during submaximal exercise and recovery.