Correlation between target reinnervation and distribution of motor axons in the injured rat sciatic nerve
Correlation between target reinnervation and distribution of motor axons in the injured rat sciatic nerve
复制标题
DOI：
10.1089/neu.2006.23.227
复制
发表时间：
2006-02-01
影响因子：
4.2
通讯作者：
Navarro, X
中科院分区：
文献类型：
--
作者：
Lago, N;Navarro, X
周围神经损伤后很少能完全恢复功能。运动功能的缺陷尤其重要，会导致瘫痪和肌肉萎缩。在不同的组中，坐骨神经被压碎或横断，直接缝合或硅胶管或胶原管修复。60天后，通过电生理和功能测试、神经形态学和免疫组织化学对胆碱乙酰转移酶（ChAT）进行评估，以标记运动轴突。缝合和管道修复导致肌肉神经再生水平相似，但明显低于神经压迫后。神经切断术后各组患者步行轨迹模式恢复较差。神经挤压后再生髓鞘纤维数量和ChAT+纤维数量与对照组相近，但经切片修复后增加。神经挤压后，正常的神经束结构和ChAT+纤维的组合得以维持，但在切除和修复后，运动纤维分散在整个神经的小再生神经束中。束状组织的丧失与运动功能恢复不足有关。因此，用ChAT免疫组化技术标记运动轴突为研究神经再生的程度和特异性提供了有用的信息。
Peripheral nerve injuries are rarely followed by complete return of function. Deficits are particularly important for motor function, resulting in paralysis and muscle atrophy. In different groups, the sciatic nerve was either crushed or transected and repaired by direct suture or by tube repair using silicone or collagen tubes. After 60 days, nerve regeneration was assessed by electrophysiological and functional tests, nerve morphology and immunohistochemistry against choline acetyltransferase (ChAT) for labeling motor axons. Suture and tube repair resulted in similar levels of muscle reinnervation, but significantly lower than after nerve crush. Recovery of walking track pattern was poor in all groups after nerve section. The numbers of regenerated myelinated fibers and of ChAT+ fibers were similar to control values after nerve crush, but increased after section and repair. The normal fascicular architecture and grouping of ChAT+ fibers were maintained after nerve crush, but lost after section and repair, where motor fibers were scattered within small regenerated fascicles throughout the nerve. The loss of fascicular organization was related to the deficient recovery of locomotor function. Thus, labeling of motor axons by ChAT immunohistochemistry provides useful information for the study of the degree and specificity of nerve regeneration.