miR-222 is involved in the regulation of genistein on skeletal muscle fiber type
miR-222 is involved in the regulation of genistein on skeletal muscle fiber type
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DOI：
10.1016/j.jnutbio.2019.108320
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发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Zhu, Li
中科院分区：
文献类型：
--
作者：
Gan, Mailin;Shen, Linyuan;Zhu, Li
在骨骼肌中，纤维类型的组成对运动表现有着深远的影响，例如耐力或力量输出。肌纤维类型的比例也与某些疾病有关，包括运动障碍、肥胖和胰岛素抵抗。染料木黄酮是一种天然雌激素，已被证明可以调节骨骼肌中的脂肪酸氧化和胰岛素敏感性。然而，金雀异黄素是否能调节骨骼肌纤维类型尚不清楚。此外，其影响骨骼肌能量代谢的机制尚不完全清楚。本研究通过体内外实验，探讨染料木素对肌纤维型转变和肌肉代谢的影响。结果表明，染料木黄酮不仅能促进小鼠骨骼肌发育，而且能增加小鼠慢肌纤维的表达。染料木黄酮改变了纤维类型的比例，促进了线粒体的形成。C2 C12成肌细胞的生物发生。有趣的是，染料木素降低了miR-222的表达，并且证明了这种microRNA靶向PGC 1 α基因。在C2 C12成肌细胞中，miR-222似乎调节纤维类型转换和线粒体生物发生。然而，这种功能显着降低染料木素治疗后。这些结果表明，miR-222可能参与了染料木素对骨骼肌纤维和肌肉代谢的调节，染料木素可能用于改善肌肉健康。(C)2019由Elsevier Inc.出版
In skeletal muscle, the composition of the fiber types has a profound impact on athletic performance, such as endurance or strength output. The proportions of muscle fiber types have also been associated with certain diseases, including dyskinesia, obesity and insulin resistance. Genistein, a natural estrogen, has been demonstrated to regulate fatty acid oxidation and insulin sensitivity in skeletal muscle. However, it is unknown whether genistein can regulate skeletal muscle fiber types. Furthermore, the mechanism of its effect on skeletal muscle energy metabolism is not entirely clear. In this study, in vivo and in vitro experiments were used to explore the effect of genistein on the muscle fiber-type transitions and muscle metabolism. The results indicated that genistein not only promotes skeletal muscle development but increases the expression of slow muscle fibers in mice as well. It was also demonstrated that genistein altered the ratios of fiber type and promoted mitochondria! biogenesis in C2C12 myoblasts. Interestingly, the expression of miR-222 was decreased by genistein, and it was demonstrated that this microRNA targets the PGC1 alpha gene. In C2C12 myoblasts, miR-222 appears to regulate fiber type conversion and mitochondrial biogenesis. However, this function was significantly reduced following genistein treatment. These results suggest that miR-222 may be involved in the regulation of genistein on skeletal muscle fiber and muscle metabolism, and genistein may be used to improve muscle health. (C) 2019 Published by Elsevier Inc.