Assessing crystallization droplets using birefringence
Assessing crystallization droplets using birefringence
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DOI：
10.1107/s0907444904003154
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发表时间：
2004-04-01
影响因子：
2.2
通讯作者：
Geday, MA
中科院分区：
文献类型：
--
作者：
Echalier, A;Glazer, RL;Geday, MA
在本文中，结晶元素的蛋白质结晶液滴含有沉淀的检测说明使用的双折射偏光显微镜技术。这种自动双折射技术的灵敏度使得能够使用传统的检查方法检测似乎是无定形的沉淀物中的微晶。用溶菌酶和葡萄糖异构酶说明了该技术。葡萄糖异构酶微晶被成功地用于接种实验和两个系统的条件进行了改进，以产生适合于X射线分析的晶体。结果是相关的高通量晶体学领域的自动晶体检测方法，以及作为一个有用的工具，详细的沉淀物分析。
In this paper, the detection of crystalline elements in protein crystallization droplets containing precipitate is illustrated using the rotating-polarizer microscope technique. The sensitivity of this automated birefringence technique enables the detection of microcrystals in a precipitate that appears to be amorphous using traditional methods of inspection. The technique is illustrated with lysozyme and glucose isomerase. Glucose isomerase microcrystals were used successfully for seeding experiments and the conditions of both of the systems were refined to produce crystals suitable for X-ray analysis. The results are relevant to the field of high-throughput crystallography as an automated crystal-detection method as well as being a useful tool for detailed precipitate analysis.