Anti-Horse Podoplanin Monoclonal Antibody PMab-219 is Useful for Detecting Lymphatic Endothelial Cells by Immunohistochemical Analysis
Anti-Horse Podoplanin Monoclonal Antibody PMab-219 is Useful for Detecting Lymphatic Endothelial Cells by Immunohistochemical Analysis
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抗马 Podoplanin 单克隆抗体 PMab-219 可用于通过免疫组织化学分析检测淋巴内皮细胞
DOI：
10.1089/mab.2018.0044
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发表时间：
2018
影响因子：
--
通讯作者：
Kaneko Mika K.
中科院分区：
文献类型：
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作者：
Kato Yukinari;Yamada Shinji;Itai Shunsuke;Kobayashi Atsushi;Konnai Satoru;Kaneko Mika K.
Podoplanin （PDPN）在淋巴内皮细胞中表达，通过c型凝集素样受体-2 （clc -2）诱导血小板聚集。该蛋白已在许多动物物种中使用特异性抗pdpn单克隆抗体（mab）进行了表征。我们最近建立了抗马PDPN （horPDPN）的单抗，命名为PMab-219。因此，在本研究中，我们研究了PMab-219是否可以检测马组织中的淋巴内皮细胞。免疫组织化学分析表明，PMab-219在马结肠组织淋巴内皮细胞中有较强的染色，这将有助于研究horPDPN在这些细胞中的功能。
Podoplanin (PDPN) is expressed in lymphatic endothelial cells, where it induces platelet aggregation through C-type lectin-like receptor-2 (CLEC-2). This protein has been characterized for a number of animal species using specific anti-PDPN monoclonal antibodies (mAbs). We recently established the mAb against horse PDPN (horPDPN) named PMab-219. Therefore, in this study, we investigated whether PMab-219 can detect lymphatic endothelial cells in horse tissues. Immunohistochemical analysis demonstrated that PMab-219 strongly stained lymphatic endothelial cells in horse colon tissues, indicating that it will be useful for investigating the function of horPDPN in these cells.