MKRN expression pattern during embryonic and post-embryonic organogenesis in rice (Oryza sativa L. var. Nipponbare)
MKRN expression pattern during embryonic and post-embryonic organogenesis in rice (Oryza sativa L. var. Nipponbare)
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水稻胚胎和胚胎后器官发生过程中的 MKRN 表达模式（Oryza sativa L. var. Nipponbare）
DOI：
10.1007/s00425-012-1828-2
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发表时间：
2013
期刊：
影响因子：
4.3
通讯作者：
Abe S.
中科院分区：
文献类型：
--
作者：
Wadekar H.B.;Sahi V.P.;Morita E.H.;Abe S.
水稻MKRN是Makorin RING指蛋白基因（MKRN）家族的一员，该家族编码一种具有多种真核生物中保守的特征性锌指基序阵列的蛋白质。利用非放射性原位杂交技术，研究了水稻MKRN基因在水稻胚胎发生、吸胀、种子和侧根发育过程中的时空表达模式。变种MKRN在胚胎早期器官发生过程中，沿沿着顶基轴和径向轴普遍表达。与早期胚胎发生相比，MKRN的表达在胚胎器官的伸长和成熟过程中有所下降，但在吸胀过程中又有所增加。在胚和胚后根和芽发育过程中发现了组织特异性和位置依赖性MKRN表达。分生组织细胞普遍表达MKRN转录本，而分化细胞显示MKRN表达逐渐减少和终止。有趣的是，在萌发后，MKRN的表达是突出的，并继续在代谢活跃，分化的韧皮部伴随细胞。在分化和分化的细胞中观察到差异表达模式。此外，MKRN表达在侧根原基及其周围细胞的各个发育阶段。MKRN的表达也被观察到后，periclinal分裂的假定pericronifounder细胞。MKRN在水稻不同发育阶段的表达模式表明，Makorin RING指蛋白基因（MKRN）在水稻顶基轴和径向轴的胚胎和胚后器官发生中起重要作用。
RiceMKRNis a member of the makorin RING finger protein gene (MKRN) family, which encodes a protein with a characteristic array of zinc-finger motifs conserved in various eukaryotes. Using non-radioactive in situ hybridization, we investigated the spatio-temporal gene expression pattern of riceMKRNduring embryogenesis, imbibition, seminal and lateral root development ofOryza sativaL. var. Nipponbare.MKRNexpression was ubiquitous during early organogenesis in the embryo along the apical–basal and radial axes. The expression ofMKRNdecreased during embryonic organ elongation and maturation compared to early embryogenesis, but increased again during imbibition. Tissue-specific and position-dependentMKRNexpression was found during embryonic and post-embryonic root and shoot development. Meristematic cells ubiquitously expressedMKRNtranscripts, while differentiating cells showed a gradual reduction and termination ofMKRNexpression. Interestingly, during post-germinationMKRNexpression was prominent and continued in the metabolically active, differentiated companion cells of the phloem. The differential expression pattern was observed both in the differentiating and differentiated cells. Also,MKRNwas expressed in the various developmental stages of the lateral root primordia and the cells surrounding them. Expression ofMKRNwas also observed after periclinal division of the presumptive pericycle founder cells. TheMKRNexpression pattern during development of various growth stages suggests an important role of makorin RING finger protein gene (MKRN) in embryonic and post-embryonic organogenesis in both apical–basal and radial developmental axes of rice.