CLONING AND CHARACTERIZATION OF BAND-3, THE HUMAN-ERYTHROCYTE ANION-EXCHANGE PROTEIN (AE1)
CLONING AND CHARACTERIZATION OF BAND-3, THE HUMAN-ERYTHROCYTE ANION-EXCHANGE PROTEIN (AE1)
复制标题
DOI：
10.1073/pnas.86.23.9089
复制
发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
LODISH, HF
中科院分区：
文献类型：
--
作者：
LUX, SE;JOHN, KM;LODISH, HF
从胎儿肝脏cDNA文库中克隆了人红细胞阴离子交换蛋白（带3或AE1）。详细分析了三个重叠的克隆，包含3637个核苷酸。它们编码了一个含有911个氨基酸的蛋白（Mr 101,791），并在人类网织红细胞RNA中检测到一个4.7千碱基的单一物种。相应的基因位于17号染色体上。该蛋白在结构上与其他阴离子交换类似，并分为三个区域：与多种膜和细胞质蛋白相互作用的亲水细胞质区域（残基1-403）；形成阴离子反转运蛋白的疏水跨膜结构域（残基404-802）；和一个功能未知的酸性c端结构域（残基883-911）。n端结构域包含几个保守片段（例如，残基57-86、102-164、219-347和375-403），其中一些可能是锚蛋白、蛋白4.1或蛋白4.2的结合位点。膜结构域是高度保守的，除了一个片段（残基543-567），它包含几个位点，可以被细胞外蛋白酶切割。基于亲水分析和丰富的可用的地形和功能数据，提出了一个模型，其中蛋白质穿过膜14次。
The human erythrocyte anion-exchange protein (band 3 or AE1) was cloned from a fetal liver cDNA library. Three overlapping clones, encompassing 3637 nucleotides, were analyzed in detail. These encode a 911-amino acid protein (Mr 101,791) and detect a single 4.7-kilobase species in human reticulocyte RNA. The corresponding gene is located on chromosome 17. The protein is similar in structure to other anion exchanges and is divided into three regions: a hydrophilic, cytoplasmic domain that interacts with a variety of membrane and cytoplasmic proteins (residues 1-403); a hydrophobic, transmembrane domain that forms the anion antiporter (residues 404-802); and an acidic, C-terminal domain of unknown function (residues 883-911). The N-terminal domain contains several conserved sections (e.g., residues 57-86, 102-164, 219-347, and 375-403), some of which may contribute to binding sites for ankyrin, protein 4.1, or protein 4.2. The membrane domain is highly conserved with the exception of a single segment (residues 543-567) that contains several sites for cleavage of the protein by extracellular proteases. Based on hydropathy analyses and the wealth of available topographical and functional data, a model is proposed in which the protein crosses the membrane 14 times.