Thermophilic and cellulolytic consortium isolated from composting plants improves anaerobic digestion of cellulosic biomass: Toward a microbial resource management approach
Thermophilic and cellulolytic consortium isolated from composting plants improves anaerobic digestion of cellulosic biomass: Toward a microbial resource management approach
复制标题
DOI：
10.1016/j.biortech.2015.04.010
复制
发表时间：
2015-08-01
影响因子：
11.4
通讯作者：
Delvigne, F.
中科院分区：
文献类型：
--
作者：
Kinet, R.;Destain, J.;Delvigne, F.
从堆肥厂中分离出纤维素分解菌群，以促进厌氧消化中遇到的初始水解步骤。纤维素降解的改善，以及沼气生产，观察与外源财团接种的文化。宏基因组学分析指出，在初始分离步骤中遇到的选择压力（纤维素作为唯一碳源）诱导的外源性聚生体的丰度较低（与OTU的数量相关）。测定的主要微生物菌株是严格厌氧的，属于梭菌纲。在纤维素厌氧降解过程中，pH下降引起了微生物种群的强烈修改。尽管事实上，丰富度和均匀度是非常弱的，外源财团是能够适应和保持纤维素降解潜力。这一重要的结果指出，简化的微生物群落可以用来提高环境生物技术中混合培养物的鲁棒性。(C)2015爱思唯尔有限公司版权所有。
A cellulolytic consortium was isolated from a composting plant in order to boost the initial hydrolysis step encountered in anaerobic digestion. Improvement of the cellulose degradation, as well as biogas production, was observed for the cultures inoculated with the exogenous consortium. Metagenomics analyses pointed out a weak richness (related to the number of OTUs) of the exogenous consortium induced by the selective pressure (cellulose as sole carbon source) met during the initial isolation steps. Main microbial strains determined were strictly anaerobic and belong to the Clostridia class. During cellulose anaerobic degradation, pH drop induced a strong modification of the microbial population. Despite the fact that richness and evenness were very weak, the exogenous consortium was able to adapt and to maintain the cellulolytic degradation potential. This important result point out the fact that simplified microbial communities could be used in order to increase the robustness of mixed cultures involved in environmental biotechnology. (C) 2015 Elsevier Ltd. All rights reserved.