Filifactor alocis manipulates human neutrophils affecting their ability to release neutrophil extracellular traps induced by PMA
Filifactor alocis manipulates human neutrophils affecting their ability to release neutrophil extracellular traps induced by PMA
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DOI：
10.1177/1753425918767507
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发表时间：
2018-05-01
期刊：
影响因子：
3.2
通讯作者：
Uriarte, Silvia M.
中科院分区：
文献类型：
--
作者：
Armstrong, Cortney L.;Klaes, Christopher K.;Uriarte, Silvia M.
中性粒细胞在牙周袋中的损伤或炎症部位起作用，以确保牙周健康和细菌病原体的清除。Filifactoralocis最近被确定为一种潜在的牙周病原体，在这项研究中，我们评估了中性粒细胞胞外陷阱（NET）的形成，响应于生物体的存在。NET形成的人中性粒细胞时，没有诱导挑战与F。alocis，不依赖于调理作用、活力、时间或细菌剂量。F. alocis也不能从TNF-α致敏的中性粒细胞诱导NET，并且不能诱导细胞外中性粒细胞弹性蛋白酶的释放。然而，当中性粒细胞被戈登链球菌或消化厌氧杆菌攻击时，观察到显著的NET诱导。alocis与S. gordonii或P. stomatis不增强或减少NET。此外，F. alocis不能影响由S. gordonii或P. stomatis。F.在用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）刺激前，gordonii或P. stomatis的研究表明，该细菌只能还原PMA，而不能还原S. gordonii或P. stomatis NET形成。这些结果表明F. alocis操纵中性粒细胞，抑制NET诱导的触发。
Neutrophils operate at the site of injury or inflammation in the periodontal pocket to ensure periodontal health and clearance of bacterial pathogens. Filifactor alocis is recently identified as a potential periodontal pathogen, and in this study, we assessed the formation of neutrophil extracellular traps (NETs), in response to the presence of the organism. NET formation by human neutrophils was not induced when challenged with F. alocis, independent of opsonization, viability, time, or bacterial dose. F. alocis also failed to induce NETs from TNF--primed neutrophils and did not induce the release of extracellular neutrophil elastase. However, significant NET induction was observed when neutrophils were challenged with Streptococcus gordonii or Peptoanaerobacter stomatis, In addition, co-infection studies revealed that the presence of F. alocis with S. gordonii or P. stomatis does not enhance or reduce NETs. Additionally, F. alocis failed to impact pre-formed NETs induced by either S. gordonii or P. stomatis. Pretreatment with F. alocis prior to stimulation with phorbol 12-myristate 13-acetate (PMA), S. gordonii, or P. stomatis revealed that the bacterium is capable of reducing only PMA but not S. gordonii or P. stomatis NET formation. These results indicate that F. alocis manipulates neutrophils, inhibiting the triggering of NET induction.