Primary structure of the human 4F2 antigen heavy chain predicts a transmembrane protein with a cytoplasmic NH2 terminus.
Primary structure of the human 4F2 antigen heavy chain predicts a transmembrane protein with a cytoplasmic NH2 terminus.
复制标题
人 4F2 抗原重链的一级结构预测具有细胞质 NH2 末端的跨膜蛋白。
DOI：
10.1016/s0021-9258(18)47972-0
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
L. Kühn
中科院分区：
文献类型：
--
作者：
S. Teixeira;S. Di Grandi;L. Kühn
人细胞表面抗原4F 2是由糖基化重链和非糖基化轻链组成的二硫键连接异二聚体。该抗原在增殖细胞上普遍表达，但仅在某些组织的静息细胞中表达。其功能被认为与细胞内Ca ~（2+）/Na ~+交换有关。我们描述了4F 2重链基因和cDNA的基因转移方法的分子克隆。从用表达4F 2抗原的第二转染子L细胞系的DNA构建的基因组λ文库中分离部分基因。来自噬菌体插入物的基因特异性探针用于分离两个全长cDNA克隆。这两个cDNA克隆都指导4F 2抗原在转染的小鼠L细胞中的表达。4F 2抗原重链基因指定具有开放阅读框的2.1-脱氢酶mRNA，编码58 kDa的529个残基蛋白。该蛋白缺乏NH 2-末端信号肽，但在其COOH-末端结构域中含有内部跨膜区和四个潜在的糖基化位点。我们预测，4F 2抗原重链是一个跨膜蛋白质的81个氨基酸的胞质NH 2末端。该抗原显示与已知蛋白质序列没有同源性。
The human cell surface antigen 4F2 is a disulfide-linked heterodimer consisting of a glycosylated heavy chain and a nonglycosylated light chain. The antigen is ubiquitously expressed on proliferating cells but only in resting cells from certain tissues. Its function has been proposed to relate to cellular Ca2+/Na+ exchange. We describe the molecular cloning of the 4F2 heavy chain gene and cDNA by a gene transfer approach. Part of the gene was isolated from a genomic lambda library constructed with DNA of a secondary transfectant L cell line that expresses 4F2 antigen. A gene-specific probe derived from the phage inserts was used to isolate two full length cDNA clones. Both cDNA clones directed the expression of 4F2 antigen in transfected mouse L cells. The 4F2 antigen heavy chain gene specifies a 2.1-kilobase mRNA with an open reading frame coding for a 529-residue protein of 58 kDa. The protein lacks an NH2-terminal signal peptide but contains an internal transmembrane-spanning region and four potential glycosylation sites in its COOH-terminal domain. We predict that the 4F2 antigen heavy chain is a transmembrane protein with a cytoplasmic NH2 terminus of 81 amino acids. The antigen shows no homology to known protein sequences.