Nanoscale control of silica morphology and three-dimensional structure during diatom cell wall formation
Nanoscale control of silica morphology and three-dimensional structure during diatom cell wall formation
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DOI：
10.1557/jmr.2006.0333
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发表时间：
2006-10-01
影响因子：
2.7
通讯作者：
Doktycz, Mitchel J.
中科院分区：
文献类型：
--
作者：
Hildebrand, Mark;York, Evelyn;Doktycz, Mitchel J.
我们提出了一种独特的方法，结合先进的成像工具的生物操作，检查硅细胞壁合成的硅藻海链藻。硅藻在纳米到微米尺度上形成复杂的3D二氧化硅结构的固有能力超过了目前的合成方法，因为它们使用了根本不同的形成过程。了解这个过程的分子细节需要识别结构中间体，并将它们的形成与所涉及的基因和蛋白质相关联。这将有助于开发可控地改变结构的方法，促进硅藻作为纳米结构材料的直接来源的使用。于T.不同的细胞壁亚结构的形成过程中观察到不同的二氧化硅形态，并确定了三种不同规模的结构组织。在所有水平上，结构形成与最终产品的最佳设计性能相关。这些结果提供了一个基准的测量和生物硅化过程的新见解，也可能有利于仿生方法。
We present a unique approach combining biological manipulation with advanced imaging tools to examine silica cell wall synthesis in the diatom Thalassiosira pseudonana. The innate capabilities of diatoms to form complex 3D silica structures on the nano- to micro-scale exceed current synthetic approaches because they use a fundamentally different formation process. Understanding the molecular details of the process requires identifying structural intermediates and correlating their formation with genes and proteins involved. This will aid in development of approaches to controllably alter structure, facilitating the use of diatoms as a direct source of nanostructured materials. In T. pseudonana, distinct silica morphologies were observed during formation of different cell wall substructures, and three different scales of structural organization were identified. At all levels, structure formation correlated with optimal design properties for the final product. These results provide a benchmark of measurements and new insights into biosilicification processes, potentially also benefiting biomimetic approaches.