Senescent changes in a neurobiological model system: Cerebellar Purkinje cell electrophysiology and correlative anatomy
Senescent changes in a neurobiological model system: Cerebellar Purkinje cell electrophysiology and correlative anatomy
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神经生物学模型系统的衰老变化：小脑浦肯野细胞电生理学和相关解剖学
DOI：
10.1016/0197-4580(80)90018-4
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发表时间：
1980
影响因子：
4.2
通讯作者：
F. Bloom
中科院分区：
文献类型：
--
作者：
J. Rogers;M. Silver;W. Shoemaker;F. Bloom
采用受试者内设计来评估小脑浦肯野神经元的年龄变化。对四组年龄分别为 3、10、20 和 28 个月的幼稚雄性 Sprague-Dawley 大鼠进行单细胞记录，以对浦肯野细胞放电模式进行电生理学评估，然后进行灌注以检测乙醛酸诱导的儿茶酚胺荧光。首先通过荧光显微镜对小脑切片进行儿茶酚胺拍照，并在 2-3 周后对脂褐质自发荧光进行定量。最后，用甲酚紫处理这些相同的组织并第三次拍照，以定量估计尼氏染色浦肯野神经元数量的年龄变化。电生理学研究揭示了年龄对许多浦肯野细胞放电参数的显着影响：特别是，老年动物中异常、放电速度非常慢的细胞数量不断增加。这些细胞表现出正常的攀爬纤维介导的爆发活动，但自发的简单尖峰放电率比正常情况低 3-5 倍。表现出最多数量的此类异常细胞的大鼠也表现出最差的尼氏染色。相反，对老年大鼠的浦肯野神经元进行良好的尼氏染色是相对正常的浦肯野细胞放电的可靠预测指标。研究发现，20 个月大时，脂褐质在浦肯野神经元中明显积累，此后显着增加。该物质的沉积几乎全部发生在体体的顶极。然而，我们的数据表明，脂褐质在浦肯野神经元中的积累，以及据报道的在下橄榄中的积累，并不是电生理功能障碍的主要原因。乙醛酸诱导的儿茶酚胺荧光没有明显的年龄变化，在单独的药理学研究中也没有发现小脑儿茶酚胺水平的任何衰老变化。
A within-subjects design was used to assess age changes in cerebellar Purkinje neurons. Four groups of naive male Sprague-Dawley rats, aged 3, 10, 20, and 28 months, underwent single cell recording for electrophysiological assessment of Purkinje cell firing patterns, followed by perfusion for glyoxylic acid induced catecholamine fluorescence. Cerebellar sections were photographed first by fluorescence microscope for catecholamines, and 2–3 weeks later for quantification of lipofuscin autofluorescence. Finally, these same tissues were treated with cresyl violet and photographed a third time to permit quantitative estimates of age changes in the number of Nissl staining Purkinje neurons. Electrophysiological studies revealed significant effects of age on a number of Purkinje cell firing parameters: in particular, increasing numbers of aberrant, very slow-firing cells were encountered in older animals. These cells showed normal climbing fiber mediated burst activity, but spontaneous simple spike firing rates 3–5 times less than normal. Rats exhibiting the highest numbers of such abnormal cells also exhibited the poorest Nissl staining. Conversely, good Nissl staining of Purkinje neurons in an old rat was a reliable predictor of relatively normal Purkinje cell firing. Lipofuscin was found to accumulate measurably in Purkinje neurons by 20 months of age, and to increase significantly thereafter. Deposition of the substance occurred almost exclusively at the apical pole of the soma. Our data suggest, however, that accumulation of lipofuscin in Purkinje neurons, as well as its reported accumulation in the inferior olive, is not a primary cause of electrophysiological dysfunction. There was no apparent age change in glyoxylic acid induced catecholamine fluorescence nor, in separate pharmacological studies, could any senescent alteration in cerebellar catecholamine levels be found.
锂和三环类抗抑郁药。
DOI：
--
发表时间：
1992
期刊：
The Psychiatric clinics of North America
影响因子：
--
作者：
Fetner,HH;Geller,B
通讯作者：
Geller,B