Heat treatment reduces oxidative stress and protects muscle mass during immobilization
Heat treatment reduces oxidative stress and protects muscle mass during immobilization
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DOI：
10.1152/ajpregu.00497.2004
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发表时间：
2005-07-01
影响因子：
2.8
通讯作者：
Dodd, SL
中科院分区：
文献类型：
--
作者：
Selsby, JT;Dodd, SL
这项研究考察了加热对固定肢体的氧化应激和肌肉质量的影响。将大鼠分为3组(n=9/组)：对照组(Con)、固定组(Im)和固定加热组(Imh)。将大鼠固定在屈足位8d。间歇组隔天升至41~41.5℃，降温前维持30min。第8天，热休克蛋白25(HSP25)和热休克蛋白72(HSP72)在IMH组较Im组显著升高，而Im组与Conn组比较差异无统计学意义。此外，单独固定化会显著增加氧化损伤，而在固定化的基础上增加加热可以显著减少氧化损伤。为了进一步确定这种保护作用的原因，对抗氧化酶活性进行了评估。Im组较Con组铜锌超氧化物歧化酶显著升高(P<0.025)，Im组较Im组降低(P<0.025)。Im组过氧化氢酶较Con组升高8%(P<；0.025)，与Im组相似。谷胱甘肽过氧化物酶、谷胱甘肽还原酶和锰超氧化物歧化酶在组间无差异。这些数据表明，加热提供了对氧化应激的保护，并在废弃萎缩期间保护了肌肉质量。这些数据还表明，抗氧化保护不是通过抗氧化酶来实现的，而热休克蛋白可能起着重要作用。
This study examined the role of heating on oxidative stress and muscle mass in immobilized limbs. Rats were divided into three groups (n = 9/group): a control group (Con), an immobilized group (Im), and an immobilized and heated group (ImH). Rats were immobilized in the plantarflexed position for 8 days. The core temperature of the ImH group was elevated to 41-41.5 degrees C on alternating days and maintained for 30 min before cooling. On day 8, both heat shock protein 25 (HSP25) and HSP72 were markedly elevated in the ImH compared with the Im group, whereas results in the Im group were not different from Con. Most notably, the ImH group had significantly larger solei compared with the Im group, which were less than those shown in the Con group. Furthermore, immobilization alone caused a significant increase in oxidative damage, and the addition of heating to immobilization significantly reduced oxidative damage. In an effort to further identify the cause of this protective effect, antioxidant enzyme activities were assessed. CuZnSOD was sharply elevated in Im compared (P < 0.025) with that in the Con and reduced in the ImH group compared with that in the Im group (P < 0.025). Catalase was elevated 8% (P < 0.025) in the Im group compared with the Con group and was similar to the ImH group. Glutathione peroxidase, glutathione reductase, and MnSOD did not differ between groups. These data indicate that heating provides protection against oxidative stress and preserves muscle mass during disuse atrophy. These data also suggest that antioxidant protection is not conferred via antioxidant enzymes, and HSPs may play an important role.