Adenovirus Lacking E1b Efficiently Induces Cytopathic Effect in HPV-16-Positive Murine Cancer Cells via Virus Replication and Apoptosis.
Adenovirus Lacking E1b Efficiently Induces Cytopathic Effect in HPV-16-Positive Murine Cancer Cells via Virus Replication and Apoptosis.
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缺乏 E1b 的腺病毒通过病毒复制和细胞凋亡有效诱导 HPV-16 阳性鼠癌细胞的细胞病变效应。
DOI：
10.1080/07357907.2018.1430812
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发表时间：
2018
影响因子：
2.4
通讯作者：
Gomez-Gutierrez,JorgeG
中科院分区：
文献类型：
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作者：
Martinez-Jaramillo,Elvis;Garza-Morales,Rodolfo;Wechman,StephenL;MontesdeOca-Luna,Roberto;Saucedo-Cardenas,Odila;Shirwan,Haval;Yolcu,Esma;McMasters,KellyM;Gomez-Gutierrez,JorgeG
条件复制腺病毒（CRAds）在小鼠癌细胞中复制不良；然而，e1b缺失的CRAds可能在HPV16-E6/ e7阳性小鼠癌细胞（TC-1）中有效复制。hpv16e7开放阅读框编码的功能类似于这些缺失的腺病毒E1蛋白。在本研究中，我们评估了e1b缺失的CRAd （Adhz60）在TC-1细胞中复制和诱导溶瘤的能力。adhz60介导的肿瘤溶解在TC-1和HeLa细胞中相似。基于E1A和六邻子的表达、感染性病毒子代的产生和adhz60诱导的细胞凋亡，可以明显地观察到病毒的高效复制。结果表明TC-1小鼠癌细胞允许Adhz60复制和溶瘤。
Conditionally replicative adenoviruses (CRAds) replicate poorly in murine cancer cells; however,E1b-deleted CRAds may replicate effectively in HPV16-E6/E7-positive murine cancer cells (TC-1). The HPV16 E7 open reading frame encodes functions analogous to these deleted adenovirus E1 proteins. In this study, anE1b-deleted CRAd (Adhz60) was evaluated for its ability to replicate and induce oncolysis in TC-1 cells. Adhz60-mediated oncolysis was similar in TC-1 and HeLa cells. Productive viral replication was evident based on expression of E1A and hexon, production of infectious virus progeny, and Adhz60-induced apoptosis. The results suggest that TC-1 murine cancer cells allow Adhz60 replication and oncolysis.