Histopathologic and biochemical changes in the muscles affected by distraction osteogenesis of the mandible
Histopathologic and biochemical changes in the muscles affected by distraction osteogenesis of the mandible
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DOI：
10.1097/00006534-199702000-00009
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发表时间：
1997-02-01
影响因子：
3.6
通讯作者：
Zeng, JB
中科院分区：
文献类型：
--
作者：
Fisher, E;Staffenberg, DA;Zeng, JB
为了研究牵张对咀嚼相关肌肉的影响，使用口腔内牵张装置延长了犬的下颌骨。在4个不同的时间间隔延长后，取咬肌和二腹肌的活组织切片，以评估10只狗咀嚼肌的时间变化。对侧肌肉的活检也取自其中6只狗，然后延长以建立对照组。每一次活检都进行了组织学和光谱分析，以检测RNA、DNA和蛋白质含量。二腹肌经历了一过性萎缩并开始牵张，但在固定48天后完全再生。咬肌最初没有变化，但只有在牵引20 mm后才显示出萎缩的迹象；在固定过程中，它继续显示出萎缩的证据。在咬肌萎缩期间，蛋白质合成显著减少；在二腹肌中没有观察到这种变化。与咬肌不同，二腹肌纤维位于与牵张向量平行的平面上。这些发现表明，在同一平面或向量(如二腹肌)上受骨骼牵张影响的任何肌肉都能适应代偿性再生和肥大。此外，那些位于不同平面的肌肉(例如，咬肌)显示出持续萎缩的证据，蛋白质合成减少。
Lengthening of the canine mandible using an intraoral distraction device was performed in order to study the effects of distraction on the associated muscles of mastication. Biopsies of the masse ter and digastric muscles were taken after lengthening at four different time intervals to assess the temporal changes in the masticatory muscles of 10 dogs. Biopsies of the muscles on the contralateral side also were taken from 6 of these dogs before lengthening to establish a control group. Each biopsy was analyzed histologically and spectophotomerically for RNA, DNA, and protein content.The digastric muscle underwent transient atrophy with initiation of distraction but regenerated completely after 48 days of fixation. The masseter muscle was unchanged initially but showed evidence of atrophy only after 20 mm of distraction; it continued to exhibit evidence of atrophy during fixation. Protein synthesis was decreased significantly during periods of atrophy in the masseter; no such change was noted in the digastric. Unlike the masseter, the digastric fibers lie in a plane parallel to the vector of distraction. These findings suggest that any muscle affected by skeletal distraction in the same plane or vector (e.g., digastric) adapts with compensatory regeneration and hypertrophy. Moreover, those muscles lying in a different plane (e.g., masseter) show persistent evidence of atrophy with decreased protein synthesis.