Role of vascular KATP channels in blood pressure variability after sinoaortic denervation in rats
Role of vascular KATP channels in blood pressure variability after sinoaortic denervation in rats
复制标题
血管 KATP 通道在大鼠窦主动脉去神经术后血压变异中的作用
DOI：
10.1038/aps.2010.195
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发表时间：
2011-02
影响因子：
8.2
通讯作者：
Fu-Ming Shen
中科院分区：
文献类型：
--
作者：
Zhong-Wei Yang;Dong-Jie Li;Chong Liu;Ping Han;Ding-Feng Su;Fu-Ming Shen
目的：探讨ATP敏感钾（K ATP）通道对去主动脉神经（SAD）大鼠血压变异性（BPV）的作用。方法：在研究前4周对雄性Sprague-Dawley大鼠进行SAD。 Kir6 的 mRNA 表达。 1、基尔6。使用实时定量聚合酶链反应测定主动脉和肠系膜动脉中的 2 和 SUR2，并使用蛋白质印迹和激光共聚焦免疫荧光测定在蛋白质水平上进行确认。建立了离体主动脉和肠系膜动脉环对腺苷和吡那地尔的浓度-反应曲线。在清醒的SAD大鼠中检测K ATP 通道开放剂和阻断剂对BPV的影响。结果：主动脉SUR2表达显着升高，而Kir6。 SAD 大鼠中的 1 低于假手术对照组。相反，在肠系膜动脉中，SUR2 和 Kir6 均存在。 SAD 大鼠中 1 的表达显着低于对照组。对于两条动脉，Kir6。 2 表达在假手术大鼠和 SAD 大鼠之间无法区分。这些发现在蛋白质水平上得到了证实。 SAD后主动脉对腺苷和吡那地尔的反应均增强，而肠系膜对腺苷的反应减弱。吡那地尔、二氮嗪、尼可地尔和格列本脲可显着降低 BPV。结论：这些研究结果表明，慢性 SAD 改变了血管 K ATP 通道的表达。这些改变影响血管反应性，并可能在慢性 SAD 大鼠的 BPV 增加中发挥作用。
Aim:To investigate the role of ATP-sensitive potassium (K ATP) channels on blood pressure variability (BPV) in sinoaortic denervated (SAD) rats.Methods:SAD was performed on male Sprague-Dawley rats 4 weeks before the study. mRNA expression of Kir6. 1, Kir6. 2 and SUR2 in aorta and mesenteric artery was determined using real-time quantitative polymerase chain reaction, and confirmed at the protein level using Western blotting and laser confocal immunofluorescence assays. Concentration-response curves of isolated aortic and mesenteric arterial rings to adenosine and pinacidil were established. Effects of K ATP channel openers and blocker on BPV were examined in conscious SAD rats.Results:Aortic SUR2 expression was significantly greater, while Kir6. 1 was lower, in SAD rats than in sham-operated controls. In contrast, in the mesenteric artery both SUR2 and Kir6. 1 expression were markedly lower in SAD rats than controls. For both arteries, Kir6. 2 expression was indistinguishable between sham-operated and SAD rats. These findings were confirmed at the protein level. Responses of the aorta to both adenosine and pinacidil were enhanced after SAD, while the mesenteric response to adenosine was attenuated. Pinacidil, diazoxide, nicorandil, and glibenclamide significantly decreased BPV.Conclusion:These findings indicate that expression of vascular K ATP channels is altered by chronic SAD. These alterations influence vascular reactivity, and may play a role in the increased BPV in chronic SAD rats.
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DOI：
10.1152/ajpregu.00047.2007
发表时间：
2007-07-01
影响因子：
2.8
作者：
Shi, Weiwei;Cui, Ningren;Jiang, Chun
通讯作者：
Jiang, Chun
DOI：
10.11501/3149427
发表时间：
1999
期刊：
The Journal of Information and Computational Science
影响因子：
--
作者：
赤尾 昌治
通讯作者：
赤尾 昌治
DOI：
10.1038/aps.2009.165
发表时间：
2009-12
期刊：
--
影响因子：
--
作者：
Jia-ling Wang;Long Wang;Zhao-tang Wu;W. Yuan;D. Su;Xin Ni;Jian-jun Yan;Wei-Zhong Wang
通讯作者：
Jia-ling Wang;Long Wang;Zhao-tang Wu;W. Yuan;D. Su;Xin Ni;Jian-jun Yan;Wei-Zhong Wang
影响因子：
--
作者：
JACOB, HJ;ALPER, RH;BRODY, MJ
通讯作者：
BRODY, MJ
DOI：
10.1111/j.1440-1681.2007.04745.x
发表时间：
2008-01-01
影响因子：
2.9
作者：
Colantuono, Giuseppe;Tiravanti, Edy Altea;Fiore, Tommaso
通讯作者：
Fiore, Tommaso