Dendritic cells suppress IgE production in B cells.
Dendritic cells suppress IgE production in B cells.
复制标题
DOI：
10.1093/intimm/dxl138
复制
发表时间：
2006-11
影响因子：
4.4
通讯作者：
Kunie Obayashi;Tomomitsu Doi;S. Koyasu
中科院分区：
文献类型：
--
作者：
Kunie Obayashi;Tomomitsu Doi;S. Koyasu
IG类转换重组（CSR）是由T细胞上的CD 40配体与B细胞上的CD 40接合而触发的。此外，最近的研究表明，树突状细胞（DC）能够通过B淋巴细胞刺激蛋白[或属于肿瘤坏死因子家族的B细胞活化因子]和增殖诱导配体直接控制B细胞的CSR。我们在这项研究中研究了DC在CSR中的调节作用，并证明DC通过两种不同的机制选择性地抑制由CD 40和IL-4刺激的B细胞的IgE产生：通过直接的细胞-细胞相互作用或通过可溶性因子，包括转化生长因子-β和IFN-γ。此外，不同的DC利用不同的机制：未成熟的骨髓来源的树突状细胞（BMDCs）和原代肺DC强烈抑制IgE CSR。另一方面，LPS诱导的成熟BMDC失去抑制IgE CSR的能力，但仍然通过降低IgE蛋白表达来抑制IgE产生。这些结果表明DC对IgE产生的新的调节功能。
Ig class switch recombination (CSR) is triggered by the engagement of CD40 on B cells by CD40 ligand on T cells. In addition, recent studies have shown that dendritic cells (DCs) are able to directly control the CSR of B cells through B lymphocyte stimulator protein [or B cell activation factor belonging to the tumor necrosis factor family] and a proliferation-inducing ligand. We examined in this study the regulatory role of DCs in CSR and demonstrate that DCs selectively suppress IgE production from B cells stimulated by CD40 and IL-4 through two different mechanisms: by direct cell-cell interaction or by soluble factors including transforming growth factor-beta and IFN-gamma. In addition, distinct DCs utilize different mechanisms: immature bone marrow-derived dendritic cells (BMDCs) and primary lung DCs strongly inhibit IgE CSR. On the other hand, LPS-induced mature BMDCs lose the ability to inhibit IgE CSR but still suppress IgE production by decreasing IgE protein expression. These results indicate novel regulatory functions of DCs on IgE production.