A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera
A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera
复制标题
棉铃虫 Helicoverpa armigera 变态过程中真核起始因子 5C 上调
DOI：
10.1186/1471-213x-9-19
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发表时间：
2009-03-08
影响因子：
--
通讯作者：
Zhao, Xiao-Fan
中科院分区：
文献类型：
--
作者：
Dong, Du-Juan;Wang, Jin-Xing;Zhao, Xiao-Fan
背景
真核生物起始因子5C（eIF5C）的直系同源物对于蛋白质翻译的起始是必需的，并且它们在发育过程中的调节不是众所周知的。
结果
从棉铃虫变态幼虫中克隆了一个编码419个氨基酸的多肽的cDNA，该多肽含有N-末端亮氨酸拉链基序和C-末端eIF5C结构域。它后来被命名为Ha-eIF5C。实时荧光定量PCR（QRT-PCR）结果显示，Ha-eIF5C在变态期间的头胸、体壁、中肠和脂肪体中有高表达。免疫组织化学显示Ha-eIF 5C分布于中肠、脂肪体和外皮的细胞质和细胞核中。Ha-eIF5C表达被20-羟基蜕皮激素（20E）上调。此外，Ha-eIF5C的转录下调后，蜕皮类固醇受体（EcR）或超气门蛋白（USP）的RNAi沉默。
结论
这些结果表明，在棉铃虫变态过程中，Ha-eIF5C被20 E通过EcR和USP转录因子上调。
Background
The orthologs of eukaryotic initiation factor 5C (eIF5C) are essential to the initiation of protein translation, and their regulation during development is not well known.
Results
A cDNA encoding a polypeptide of 419 amino acids containing an N-terminal leucine zipper motif and a C-terminal eIF5C domain was cloned from metamorphic larvae of Helicoverpa armigera. It was subsequently named Ha-eIF5C. Quantitative real-time PCR (QRT-PCR) revealed a high expression of the mRNA of Ha-eIF5C in the head-thorax, integument, midgut, and fat body during metamorphosis. Immunohistochemistry suggested that Ha-eIF5C was distributed into both the cytoplasm and the nucleus in the midgut, fat body and integument. Ha-eIF5C expression was upregulated by 20-hydroxyecdysone (20E). Furthermore, the transcription of Ha-eIF5C was down regulated after silencing of ecdysteroid receptor (EcR) or Ultraspiracle protein (USP) by RNAi.
Conclusion
These results suggested that during metamorphosis of the cotton bollworm, Ha-eIF5C was upregulated by 20E through the EcR and USP transcription factors.