Isolation, Physiological Characterization of Bacteriophages from Enhanced Biological Phosphorus Removal Activated Sludge and Their Putative Role
Isolation, Physiological Characterization of Bacteriophages from Enhanced Biological Phosphorus Removal Activated Sludge and Their Putative Role
复制标题
DOI：
--
复制
发表时间：
2004
影响因子：
2.8
通讯作者：
Sang Hyon Lee;H. Satoh;H. Katayama;T. Mino
中科院分区：
文献类型：
--
作者：
Sang Hyon Lee;H. Satoh;H. Katayama;T. Mino
本研究旨在表征从执行强化生物除磷（EBPR）的活性污泥中分离出的噬菌体，以了解噬菌体宿主系统与细菌群落之间的相互作用。从 EBPR 活性污泥中分离出 16 个细菌分离株 (E1-E16) 作为宿主菌株，用于噬菌体分离。根据噬菌斑大小，从 EBPR 活性污泥的过滤上清液中获得了 40 个噬菌体（E4 上有 2 个噬菌斑，E8 上有 4 个噬菌斑，E10 上有 11 个噬菌斑，E14 上有 5 个噬菌斑，E16 上有 18 个噬菌斑）。除其自身的宿主菌株外，每种噬菌体均未在本研究中测试的细菌菌株上形成任何噬菌斑，这表明噬菌体具有较窄的宿主特异性。然而，在这项研究中获得的 40 个噬菌体中有 14 个即使对它们自己的宿主细菌也失去了毒力。所有裂解性噬菌体均表现出相似的一步生长模式，并且在宿主细菌细胞中具有较长的潜伏期（约9小时）来繁殖噬菌体颗粒。另一方面，它们可能的爆发大小（每个宿主细胞 6 至 48 个）足够大，足以主动裂解宿主细菌细胞。因此，噬菌体也是EBPR活性污泥中微生物群落的重要成员，并且裂解噬菌体通过裂解直接减少EBPR活性污泥中宿主菌群的种群规模。
This study aims at characterizing the bacteriophages isolated from activated sludge performing enhanced biological phosphorous removal (EBPR) to understand the interactions between the phage-host system and bacterial community. Sixteen bacterial isolates (E1-E16) were isolated as host bacterial strains from EBPR activated sludge for phage isolation. Forty bacteriophages based on their plaque sizes (2 plaques on E4, 4 on E8, 11 on E10, 5 on E14, 18 on E16) were obtained from filtered supernatant of the EBPR activated sludge. Each bacteriophage did not make any plaque on bacterial strains tested in this study except on its own host bacterial strain, respectively, indicating that the bacteriophages are with narrow host specificity. However, fourteen of the forty bacteriophages obtained in this study lost their virulent ability even on their own host bacteria. All of the lytic phages showed similar one-step growth patterns and had long latent period (about 9 hours) to reproduce their phage particles in their host bacterial cells. On the other hand, their probable burst sizes (6 to 48 per host cell) were large enough to actively lyse their host bacterial cells. Therefore, it could be implied that bacteriophages are also important members of the microbial community in EBPR activated sludge, and lytic phages directly decrease the population size of their host bacterial groups in EBPR activated sludge by lysis.