EFFECT OF CAPSULE PERMEABILITY ON GROWTH OF CHO CELLS IN EUDRAGIT-RL MICROCAPSULES - USE OF FITC-DEXTRAN AS A MARKER OF CAPSULE QUALITY
EFFECT OF CAPSULE PERMEABILITY ON GROWTH OF CHO CELLS IN EUDRAGIT-RL MICROCAPSULES - USE OF FITC-DEXTRAN AS A MARKER OF CAPSULE QUALITY
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DOI：
10.1016/0142-9612(89)90087-2
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发表时间：
1989-09-01
期刊：
影响因子：
14
通讯作者：
SEFTON, MV
中科院分区：
文献类型：
--
作者：
BROUGHTON, RL;SEFTON, MV
采用界面沉淀法将中国仓鼠卵巢成纤维细胞包裹在Eudradit RL中。在包裹后，它们生长到充满胶囊，最终也生长在胶囊之外。通过将FITC-葡聚糖(相对分子质量为150 000)与细胞一起包裹，可以证明那些在包裹后不久失去荧光的胶囊中的细胞生长更快。这些数据被解释为表明完整的Eudradit RL胶囊膜对重要营养物质或特定代谢物具有显著的扩散阻力，从而减缓甚至阻止生长。
Chinese hamster ovary fibroblast cells were encapsulated in Eudragit RL by interfacial precipitation. After encapuslation, they grew to fill the capsules, eventually growing outside the capsules as well. By encapsulating FITC-dextran (MW 150 000) along with the cells, it was possible to show that cell growth was faster in those capsules which lost fluorescence soon after encapsulation. These data were interpreted to signify that the intact Eudragit RL capsule membrane offered significant diffusion resistance to vital nutrients or particular metabolites, slowing or even preventing growth.