MYBA2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine.
MYBA2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine.
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DOI：
10.4238/gmr15048922
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
T. Niu;Z. Gao;P. F. Zhang;X. J. Zhang;M. Gao;W. Ji;W. Fan;P. Wen
中科院分区：
文献类型：
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作者：
T. Niu;Z. Gao;P. F. Zhang;X. J. Zhang;M. Gao;W. Ji;W. Fan;P. Wen
MYBA2转录因子(Myb相关基因)影响葡萄果实的着色，在花青素的生物合成中起重要作用。从欧洲葡萄品种中克隆了MYBA2基因。制备了赤霞珠和抗VvmybA2多克隆抗体。VvmybA2基因在叶片、茎、花、芽、根、浆果和卷须等7个组织中的转录和翻译水平上均有表达。结果表明，VvmybA2在果实中的表达约为其他六种组织的11倍，并在盛花后60d开始迅速增加，在80d时达到最大值。此外，花青素含量和UDP-葡萄糖：类黄酮-3-O-葡萄糖基转移酶(UFGT)基因的表达水平在盛花后60d迅速上升。相关分析表明，在果实发育过程中，VvmybA2基因的转录和翻译表达水平不仅与UFGT和DFR基因呈显著正相关，而且与花青素含量呈显著正相关。这些结果表明，VvmybA2不仅可以调节UFGT和DFR的转录，而且还参与了葡萄果实中与颜色决定相关的UFGT基因的表达。
MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and tendril) and during the berry development stage at transcriptional and translational levels, respectively. The results indicated that the expression of VvmybA2 was approximately 11-fold higher in the berry than that in the other six tissues, and increased rapidly from 60 days after full bloom reaching a maximum on day 80. Furthermore, both the anthocyanin content and UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT) gene expression levels increased rapidly 60 days after full bloom. Moreover, correlation analysis indicated that the transcriptional and translational expression levels of the VvmybA2 gene were significantly positively correlated with not only UFGT and DFR genes but also with the anthocyanin content during berry development. These results suggested that VvmybA2 could not only regulate the transcription of both UFGT and DFR but also is involved in the expression of the UFGT gene associated with color determination in grape berries.