Interaction of Bartonella henselae with endothelial cells results in rapid bacterial rRNA synthesis and replication
Interaction of Bartonella henselae with endothelial cells results in rapid bacterial rRNA synthesis and replication
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DOI：
10.1046/j.1462-5822.2000.00072.x
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发表时间：
2000-10-01
影响因子：
3.4
通讯作者：
Autenrieth, IB
中科院分区：
文献类型：
--
作者：
Kempf, VAJ;Schaller, M;Autenrieth, IB
汉赛巴尔通体是一种生长缓慢的微生物，是引起人类杆菌性血管瘤病的病原体。具有内皮细胞的henselae可能影响细菌生长。为了这个目的，细菌rRNA生产和核糖体含量测定荧光原位杂交（FISH）使用rRNA靶向荧光标记的寡核苷酸探针。B。在琼脂平板上生长的汉赛氏菌通过FISH检测未检测到rRNA含量，而B.与内皮细胞共培养的henselae在接种后的第一个18小时内显示出rRNA产量的快速增加。rRNA合成的增加被类似于1000倍的细胞内细菌复制所抑制，而在琼脂基上生长的细菌在培养的前48小时内仅显示出类似于10倍的复制。用多聚甲醛预处理宿主细胞可阻止B的粘附、侵袭、细胞内复制和细菌rRNA合成。亨瑟莱相反，放线菌酮抑制宿主细胞蛋白质合成不影响细菌的粘附和侵袭，但阻止细胞内复制，虽然细菌rRNA含量增加。细胞松弛素D对肌动蛋白聚合的抑制并不影响B的粘附、侵袭、rRNA含量增加或细胞内复制。亨瑟莱这些结果表明，rRNA合成和复制的B。henselae由具有完整从头蛋白质合成的活宿主细胞促进。
Bartonella henselae is a slow-growing microorganism and the causative pathogen of bacillary angiomatosis in man. Here, we analysed how interaction of B. henselae with endothelial cells might affect bacterial growth. For this purpose, bacterial rRNA production and ribosome content was determined by fluorescence in situ hybridization (FISH) using rRNA-targeted fluorescence-labelled oligonucleotide probes. B. henselae grown on agar plates showed no detectable rRNA content by means of FISH, whereas B. henselae co-cultured with endothelial cells showed a rapid increase of rRNA production within the first 18 h after inoculation. The increased rRNA synthesis was paralleled by a similar to 1000-fold intracellular bacterial replication, whereas bacteria grown on agar base showed only a similar to 10-fold replication within the first 48 h of culture. Pretreatment of host cells with paraformaldehyde prevented adhesion, invasion, intracellular replication and bacterial rRNA synthesis of B. henselae. In contrast, inhibition of host cell protein synthesis by cycloheximide did not affect bacterial adhesion and invasion, but prevented intracellular replication although bacterial rRNA content was increased. Inhibition of actin polymerization by cytochalasin D did not affect adhesion, invasion, increased rRNA content or intracellular replication of B. henselae. These results demonstrate that rRNA synthesis and replication of B. henselae is promoted by viable host cells with intact de novo protein synthesis.