Pathogenicity and transmission studies of H7N7 avian influenza virus isolated from feces of magpie origin in chickens and magpie
Pathogenicity and transmission studies of H7N7 avian influenza virus isolated from feces of magpie origin in chickens and magpie
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DOI：
10.1016/j.vetmic.2009.09.027
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发表时间：
2010-03-24
影响因子：
3.3
通讯作者：
Lee, Youn-Jeong
中科院分区：
文献类型：
--
作者：
Kim, Min-Chul;Jeong, Ok-Mi;Lee, Youn-Jeong
h7n9禽流感病毒[A/喜鹊/Kr/YJD174/07 (H7N7)]；Mp/Kr/07病毒从韩国西北地区（Youngjongdo）喜鹊粪便中分离得到，经静脉致病指数和裂解位点氨基酸序列鉴定为低致病性。遗传分析显示，Mp/Kr/07病毒基因组与从永宗岛以北5公里江华野鸭中分离的另外两种H7N7病毒基因组相同，并被归为h7亚型欧亚谱系，与韩国最近分离的两种家鸭具有最高的相似性。在鸡和喜鹊的体内研究中，Mp/Kr/07病毒虽然没有引起任何临床症状和组织学改变，但可以在鸡口咽和泄殖腔中复制，并有效地传播给接触鸡。然而，该病毒在喜鹊的口咽部被限制性地鉴定出来，并没有通过直接接触传播给喜鹊。这些结果表明，喜鹊不是流感病毒的生物放大器，因此作为中间宿主在病毒传播中发挥的作用很小。(C) 2009 Elsevier B.V.版权所有
An H7N7 avian influenza virus [A/Magpie/Kr/YJD174/07 (H7N7); Mp/Kr/07 virus] was isolated from magpie feces in the north-western area (Youngjongdo) of South Korea and identified as low pathogenicity by intravenous pathogenic index and amino acid sequence of cleavage site. In genetic analysis, the genome of the Mp/Kr/07 virus was the same as those of two other H7N7 viruses isolated from the Mallard ducks in Ganghwa, 5 km north of Youngjongdo, and grouped under the H7-subtype Eurasian linage with the highest similarity to recent two domestic duck isolates in South Korea. In vivo studies of the chickens and magpies, the Mp/Kr/07 virus, though did not caused any clinical signs with histological changes, could replicate in the oropharynx and cloaca of the chickens and was efficiently transmitted to contact chickens. However, the virus was restrictively identified in oropharynx of the magpies and was not spread to magpies by direct contact. These results suggest that magpie are not a biological amplifier of influenza virus and thus play a minimal role in virus transmission as intermediate host. (C) 2009 Elsevier B.V. All rights reserved.